Methylation of death-associated protein kinase in ovarian carcinomas

International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society
Y CollinsK Odunsi

Abstract

Death-associated protein (DAP) kinase is a serine/threonine kinase that plays an integral role in apoptosis and metastasis. The purpose of our study was to determine the methylation status of DAP kinase in ovarian carcinomas. Thirty-one patients with histologically confirmed epithelial ovarian cancers treated at Roswell Park Cancer Institute, Buffalo, New York, between 1987 and 1999 were studied. Sixty-two samples were examined for DAP kinase methylation status: 1 normal human genomic DNA sample from a healthy individual, 1 transformed normal surface ovarian epithelial cell line (IOSE, from Dr Nancy Auersperg, Vancouver, Canada), 2 ovarian carcinoma cell lines (OVCAR3 and A2780), 1 ovarian serous cystadenoma, and 30 ovarian carcinomas. Additionally, peripheral blood DNA was examined from the patients with the serous cystadenoma and ovarian carcinomas. Methylation-specific polymerase chain reaction was performed using primers designed for the unmethylated and methylated promoter regions. The DAP kinase gene was unmethylated in both the normal human genomic DNA sample and the transformed normal surface epithelial ovarian cell line. The two ovarian cancer cell lines were methylated. In the 30 patients with malignant disease, methy...Continue Reading

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Citations

Jan 17, 2012·Cancer Letters·Brian S Gloss, Goli Samimi
Feb 28, 2007·The Journal of Biological Chemistry·Liguo ZhangPatricia J Gallagher
Aug 13, 2010·Biomarkers in Medicine·Leonel Maldonado, Mohammad Obaidul Hoque
Dec 4, 2012·International Journal of Molecular Sciences·Vladimir KashubaEugene Zabarovsky
Dec 7, 2013·Apoptosis : an International Journal on Programmed Cell Death·Yide HuangYao Lin
Mar 14, 2013·Expert Opinion on Medical Diagnostics·Jonathan G BijronPaul J van Diest
Feb 1, 2008·Gynecologic Oncology·Caroline A BartonPhilippa M O'Brien
Mar 20, 2020·International Journal of Cancer. Journal International Du Cancer·Alka SinghManisha Sachan
Nov 2, 2013·Oncology Letters·Chiharu UekuriHiroshi Kobayashi
Aug 24, 2021·Frontiers in Molecular Biosciences·Qingshui WangYao Lin

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