Methylation of Integrin α4 and E-Cadherin Genes in Human Prostate Cancer

Pathology Oncology Research : POR
Z Mostafavi-PourA Monabati

Abstract

Prostate cancer is the second most common malignancy in men worldwide. Abnormal epigenetic alterations such as DNA methylation and histone modification play an important role in tumor initiation, progression and regulation of cancer-related genes such as integrin α4 and E-cadherin. Expression of these genes was determined by semi-quantitative reverse transcriptase-PCR in prostate cancer cell lines, DU145 and PC3, before and after treatment with 5-aza-2-deoxycytidine and trichostatin A. Laser capture microdissection microscopy was used to obtain exclusively affected epithelial cells from prostate gland biopsies of 30 patients with prostate cancer and 40 with benign prostate hyperplasia. DNA bisulfite modifications followed by methylation-specific PCR were used to evaluate the promoter methylation status of E-cadherin and α4 integrin genes in extracted DNA from patients and aforementioned cell lines. The integrin α4 promoter in DU145 was fully methylated, whereas in PC3 cells, partial methylation was detected. E-cadherin was expressed in both cell lines; trichostatin A and 5-aza-2-deoxycytidine treatment had no effect on E-cadherin expression, however the combined treatment of both drugs or 5-aza-2-deoxycytidine alone increased i...Continue Reading

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Citations

Feb 8, 2018·Molecular Biology Reports·Zohreh Mostafavi-PourTahereh Talaei-Khozani
Jan 3, 2020·Critical Reviews in Clinical Laboratory Sciences·Majid Ghayour-MobarhanMeysam Moghbeli
Dec 19, 2019·Frontiers in Genetics·Carlos Eduardo Fonseca-AlvesRenee Laufer-Amorim
Oct 29, 2018·Pathology Oncology Research : POR·Marcela ChmelarovaVladimir Palicka
Jan 28, 2021·Scientific Reports·Vladimir V StrelnikovOlga A Simonova
Apr 23, 2021·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Iman AkhlaghipourMeysam Moghbeli

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