PMID: 1204616Nov 1, 1975Paper

Methylation of ribosomal-precursor RNA, synthesized in vitro, by isolated rat-liver nucleoli

European Journal of Biochemistry
I GrummtJ M Slack

Abstract

Nucleoli isolated from rat liver were incubated for synthesis of RNA in vitro in the presence or absence of S-adenosyl [3H] methionine. The results obtained indicate that neither the rate of RNA synthesis not the processing of pre-ribosomal RNA was changed if methylation was allowed to take place. The methylation process acts on the RNA most recently synthesized, rather than on the bulk of the RNA already present in the nucleoli. The reaction seems to occur faithfully both quantitatively and qualitatively. It is calculated that 104 mol methyl groups were incorporated per mol of newly synthesized 45-S RNA. Methylation of the ribose rather than the bases predominated. The pattern of alkali-stable oligonucleotides of RNA methylated in vitro was analyzed and found to correspond closely to that of ribosomal RNA labelled in vivo.

References

Sep 1, 1975·European Journal of Biochemistry·I Grummt
Nov 12, 1970·Biochimica Et Biophysica Acta·M C LiauR B Hurlbert
Mar 15, 1974·European Journal of Biochemistry·J M Slack, U E Loening
Aug 10, 1973·Nature·M Salim, B E Maden
Mar 15, 1974·European Journal of Biochemistry·J M Slack, U E Loening
Jul 2, 1973·European Journal of Biochemistry·I Grummt, R Lindigkeit
Jul 1, 1969·Physiological Reviews·J L Starr, B H Sells
Mar 1, 1970·Archives of Biochemistry and Biophysics·L A Culp, G M Brown
May 21, 1968·Biochimica Et Biophysica Acta·M Muramatsu, T Fujisawa
Feb 1, 1969·European Journal of Biochemistry·S T JacobH N Munro
Mar 1, 1969·Archives of Biochemistry and Biophysics·A A GribnauH Bloemendal
Jul 1, 1964·Canadian Journal of Biochemistry·H SINGH, B G LANE
Mar 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·M C LIAUR B HURLBERT
Nov 28, 1966·Journal of Molecular Biology·H Greenberg, S Penman

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Citations

Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·N R BallalH Busche
Sep 15, 1977·European Journal of Biochemistry·I Grummt
Jan 1, 1976·Progress in Biophysics and Molecular Biology·A A Hadjiolov, N Nikolaev
Dec 21, 2018·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Lilia AyadiYuri Motorin
Jan 1, 1984·Journal of Ultrastructure Research·J CerveraJ Renau-Piqueras

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