Methylation of sulfhydryl groups: a new function for a family of small molecule plant O-methyltransferases.

The Plant Journal : for Cell and Molecular Biology
Heather CoinerJoachim Schröder

Abstract

In plants, type I and II S-adenosyl-l-methionine-dependent O-methyltransferases (OMTs) catalyze most hydroxyl group methylations of small molecules. A homology-based RT-PCR strategy using Catharanthus roseus (Madagascar periwinkle) RNA previously identified six new type I plant OMT family members. We now describe the molecular and biochemical characterization of a seventh protein. It shares 56-58% identity with caffeic acid OMTs (COMTs), but it failed to methylate COMT substrates, and had no activity with flavonoids. However, the in vitro incubations revealed unusually high background levels without added substrates. A search for the responsible component revealed that the enzyme methylated dithiothreitol (DTT), the reducing agent added for enzyme stabilization. Unexpectedly, product analysis revealed that the methylation occurred on a sulfhydryl moiety, not on a hydroxyl group. Analysis of 34 compounds indicated a broad substrate range, with a preference for small hydrophobic molecules. Benzene thiol (Km 220 microm) and furfuryl thiol (Km 60 microm) were the best substrates (6-7-fold better than DTT). Small isosteric hydrophobic substrates with hydroxyl groups, like phenol and guaiacol, were also methylated, but the activities...Continue Reading

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Citations

Mar 11, 2008·The Plant Cell·Jun MurataVincenzo De Luca
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Jun 11, 2021·The Science of the Total Environment·Jing Jing Zhang, Hong Yang
Jan 6, 2022·PloS One·Gorkha Raj Giri, Priti Saxena

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