Methylation of the claudin‑3 promoter predicts the prognosis of advanced gastric adenocarcinoma

Oncology Reports
Zhenzhen ZhangSheng Zhang

Abstract

Claudin‑3 expression is associated with gastric cancer progression, but the role of epigenetic modifications remains unclear. We investigated methylation of the claudin‑3 promoter and expression profiles in gastric adenocarcinoma and their associations with clinicopathological characteristics and prognosis of the patients. A total of 122 patients with advanced gastric cancer [stage IIB‑IV, with lymph node (LN) metastasis] were enrolled. Each patient provided 4 tissue samples: normal gastric epithelium, intestinal metaplasia, primary tumor and metastatic LN. Claudin‑3 protein expression was examined by immunohistochemistry. Claudin‑3 promoter methylation was determined by methylation‑specific PCR and verified by bisulfite sequencing PCR. Claudin‑3 mRNA expression was measured by real‑time PCR in a subset of cases, and its correlation with protein expression was analyzed using Spearman correlation. Kaplan‑Meier survival analysis was performed (log‑rank test). Factors associated with survival were identified by Cox regression. The strong expression rate of claudin‑3 in intestinal metaplasia, primary tumor, metastatic LN and normal gastric epithelium was 91.8, 58.2, 30.3 and 13.9%, respectively. The promoter hypermethylation rate i...Continue Reading

Citations

Jan 7, 2020·Journal of Biomedical Materials Research. Part a·Libo ZhouYupeng Chen
Apr 21, 2020·Arkhiv patologii·N V DanilovaP G Malkov

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Methods Mentioned

BETA
acetylation
electrophoresis
PCR

Software Mentioned

Quantification Tool for Methylation
SPSS

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