PMID: 7538324Jan 1, 1994Paper

Methylation of the conserved A1518-A1519 in Escherichia coli 16S ribosomal RNA by the ksgA methyltransferase is influenced by methylations around the similarly conserved U1512.G1523 base pair in the 3' terminal hairpin

Biochimie
L J FormenoyJ Ofengand

Abstract

An in vitro system developed for the site-specific mutagenesis of 16S rRNA of Escherichia coli ribosomes was used to make five mutations around the highly conserved U1512.G1523 base pair in the 3' terminal hairpin. Each of the mutant RNAs was reconstituted with a complete mixture of 30S proteins to yield 30S ribosomal particles, which were tested for the ability of the ksgA methylase to form m6(2)A1518 and m6(2)A1519. Dimethylation of A1518 and A1519 in the hairpin loop was inhibited 20-80% by the mutations. The results indicate that G1523 and C1524 in the stem are important determinants for the dimethylation of A1518 and A1519 in the loop. Either the enzyme recognition region extends that far or the effect of mutations in the stem are propagated in some manner to the loop. The conserved U.G base pair does not of itself appear to play a major role in ksgA methylase recognition.

References

Jan 11, 1991·Progress in Nucleic Acid Research and Molecular Biology·M Chastain, I Tinoco
Jan 1, 1991·Annual Review of Biochemistry·H F Noller
Sep 11, 1991·Nucleic Acids Research·P R CunninghamJ Ofengand
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Citations

Jan 28, 2005·Antimicrobial Agents and Chemotherapy·Courtney E MausThomas M Shinnick
Sep 25, 2007·Journal of Bacteriology·Koichi InoueMasayori Inouye
May 3, 2011·Annual Review of Biochemistry·Zahra ShajaniJames R Williamson
Apr 25, 2007·Advances in Genetics·Kathleen L Triman
Oct 1, 2020·Cells·Ivan LaptevPetr Sergiev
Aug 9, 2020·Frontiers in Genetics·M Isabel G Lopez SanchezJoanna Rorbach
Jan 10, 2021·Trends in Cell Biology·Priyanka MaitiRicarda Richter-Dennerlein
Jan 13, 2021·Nucleic Acids Research·Robert KnüppelSébastien Ferreira-Cerca
Aug 10, 2021·Frontiers in Microbiology·Paola Londei, Sébastien Ferreira-Cerca

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