Methylation profile of genes on the human X chromosome

Epigenetics : Official Journal of the DNA Methylation Society
Ashwin Kelkar, Deepti Deobagkar

Abstract

X chromosome inactivation occurs in female mammals for the purpose of equalisation of dosage of X linked genes between the two sexes. In eutherian mammals, one of the two copies of the X chromosome present in female individuals is silenced. Epigenetic modifications of both DNA and histones have been implicated to play a crucial role in this inactivation phenomenon. In this work, we have employed a novel method published earlier by us, to assess the DNA methylation levels of genes on the inactive X chromosome in the human system. We have used genomic DNA from cells with the following karyotype namely, 47,XXX and 45,X to compare methylation levels from the active and inactive X. We report differential methylation of genes from the active and the inactive X chromosome with higher number of methylated genes being present on the inactive X chromosome. Our work has also led to identification of motifs that show a significant similarity to microRNA sequences which are enriched in methylated regions specific to the inactive X.

Citations

Sep 8, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Yan LiQianjin Liang
Sep 10, 2014·Birth Defects Research. Part A, Clinical and Molecular Teratology·Paul RamaekersYves Jacquemyn
Nov 26, 2013·Veterinary Immunology and Immunopathology·Benjamin B Green, David E Kerr
Mar 31, 2015·World Journal of Pediatrics : WJP·Zi-Yan JiangChao-Chun Zou
Oct 1, 2016·Scientific Reports·Christian TrolleClaus Højbjerg Gravholt
Apr 11, 2018·Clinical Epigenetics·Francisco Álvarez-Nava, Roberto Lanes
Dec 12, 2017·Briefings in Functional Genomics·Shriram N Rajpathak, Deepti D Deobagkar
Feb 28, 2019·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·Mette ViuffClaus H Gravholt
Jun 3, 2020·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·Anne SkakkebaekClaus H Gravholt
Jun 4, 2020·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·Morten Muhlig NielsenClaus Højbjerg Gravholt
Sep 11, 2021·In Vitro Cellular & Developmental Biology. Animal·Vinayak S BiradarDeepti D Deobagkar

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