PMID: 11932355Apr 5, 2002Paper

Methylation status and expression of human telomerase reverse transcriptase mRNA in relation to hypermethylation of the p16 gene in colorectal cancers as analyzed by bisulfite PCR-SSCP

Japanese Journal of Clinical Oncology
Kiyoaki NomotoTadao Kakizoe

Abstract

The expression level of human telomerase reverse transcriptase (hTERT) is correlated with telomerase activity and is expressed at high levels in malignant tumors. It is of interest whether expression of hTERT is regulated by methylation of the CpG island in the promoter of the hTERT gene. We examined hTERT expression and methylation status of the hTERT and other genes including p16. We analyzed methylation status by bisulfite treatment and polymerase chain reaction with single-strand conformation polymorphism analysis (PCR-SSCP) and expression of the hTERT by RT-PCR, in 13 cancer cell lines, eight white blood cell samples and 24 colorectal cancer tissues. In the cancer cell lines, hTERT was expressed and the CpG island of the hTERT promoter was methylated. Most colorectal cancer tissues showed similar results. The promoter of hTERT was methylated in six cases, partially methylated in 17 cases and unmethylated in one case. All cases with methylation of hMLH1 or p16 also showed methylation of hTERT; however, some of the cases lacking p16 methylation also had hTERT methylation. Increased expression of hTERT is related to hypermethylation of hTERT in colorectal cancerous tissues as well as some cancer cell lines and disconcordant w...Continue Reading

Citations

Apr 22, 2004·Gynecologic Oncology·Andreas WidschwendterMartin Widschwendter
Jun 18, 2003·Biochemical and Biophysical Research Communications·Nadejda G LopatinaTrygve O Tollefsbol
Jul 28, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Marta GladychBlazej Rubis
Nov 17, 2009·BMC Genetics·Jeffrey N BryanCharles W Caldwell
Jun 11, 2010·BMC Cancer·Jillian de WildeRenske D M Steenbergen
Feb 6, 2016·Cellular and Molecular Life Sciences : CMLS·Semih Can AkincilarVinay Tergaonkar
Jul 10, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Ugur DeligezerNejat Dalay
Nov 16, 2004·Biochemical and Biophysical Research Communications·Isabelle Guilleret, Jean Benhattar
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Oct 7, 2009·The Journal of Pathology·Stéphanie BougelJean Benhattar
Aug 1, 2012·Oncology Letters·Cristina Valls-BautistaJean Benhattar
Aug 3, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Kazuya HaraguchiKazuhiro Tominaga
May 30, 2020·Cellular Oncology (Dordrecht)·David EsopiSrinivasan Yegnasubramanian
Feb 28, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Stéphanie BougelJean Benhattar

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