PMID: 7545404Sep 1, 1995Paper

Methylation status of CpG sites in the mouse and human CFTR promoters

DNA and Cell Biology
E Denamur, F F Chehab

Abstract

To determine whether a relationship exists between DNA methylation and CFTR gene expression, we investigated the methylation status of CpG sites in the mouse and human CFTR promoters. Tissues and previously characterized cell lines that vary with respect to CFTR expression were selected for analysis using the methylation sensitive restriction endonuclease Hha I. We find that CpG sites are not methylated in high and low CFTR-expressing cell lines, whereas in the very low or non-CFTR-expressing cell lines, the CpG sites are partially or completely methylated. However, none of these sites were methylated in any of the tissues examined irrespective of the state of CFTR expression. Therefore, we conclude that the CFTR promoter belongs to the class of CpG-rich promoters in which the associated CpG sites are not methylated in tissues and that an inverse correlation between methylation and CFTR expression can only be found in cell lines.

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Jul 1, 1994·Human Molecular Genetics·E Denamur, F F Chehab
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Citations

Oct 27, 2009·American Journal of Respiratory Cell and Molecular Biology·Marzena A LewandowskaAnn Harris
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·R E EllsworthE D Green
Feb 24, 2015·Biochimica Et Biophysica Acta·Halima Ouadid-AhidouchAhmed Ahidouch
May 18, 2010·Clinical Chemistry·Thomas von KanelSabina Gallati

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