Methylation status of the Ep-CAM promoter region in human breast cancer cell lines and breast cancer tissue

Cancer Letters
Gilbert SpizzoAlbert Amberger

Abstract

We examined the methylation status of the Ep-CAM promoter region of human breast cancer cell lines and breast cancer tissue using MethyLight technology and bisulfite sequencing. We found the promoter of Ep-CAM-negative breast cancer cell lines Hs 578T to be methylated to a higher degree as compared to positive cell lines MCF-7. Demethylation of cell lines was performed using 5-aza-2'-deoxycytidine. Ep-CAM RNA and protein expression could be partially restored by treating cells with 5-Aza-2'-deoxycytidine. In most primary breast cancer tissue, methylation of the Ep-CAM gene could be detected at a low level and no correlation was found with Ep-CAM protein expression in tumour tissue. Taken together, these data suggest that methylation of the Ep-CAM promoter is not a crucial mechanism for regulation of Ep-CAM expression in breast cancer. Thus, most important regulatory mechanisms have to be supposed in vivo.

References

Mar 15, 1990·International Journal of Cancer. Journal International Du Cancer·H DurbinW F Bodmer
Nov 15, 1986·International Journal of Cancer. Journal International Du Cancer·N K SpurrW F Bodmer
Oct 1, 1994·Cell Adhesion and Communication·S V LitvinovS O Warnaar
Apr 1, 1994·The Journal of Cell Biology·S V LitvinovS O Warnaar
May 18, 1999·Experimental Cell Research·L M MielnickiB B Asch
Dec 22, 1999·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·M BalzarS V Litvinov
Mar 29, 2000·Nucleic Acids Research·C A EadsP W Laird
Apr 12, 2002·International Journal of Cancer. Journal International Du Cancer·Gilbert SpizzoGünther Gastl
Aug 3, 2002·Oncogene·Martin Widschwendter, Peter A Jones
Apr 16, 2003·Cancer Immunology, Immunotherapy : CII·Linda M ThurmondVincent C Knick
Nov 30, 2004·Breast Cancer Research and Treatment·Gilbert SpizzoGuenther Gastl

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Citations

Sep 15, 2010·Carcinogenesis·Bernardina T F van der GunMarianne G Rots
Mar 19, 2011·Journal of Clinical Pathology·Gilbert SpizzoPhilip Went
Feb 2, 2011·BMC Cancer·Johanna M GostnerGilbert Spizzo
May 15, 2008·International Journal of Cancer. Journal International Du Cancer·Heidi FieglMartin Widschwendter
Feb 6, 2015·International Journal of Oncology·Mei-Ying LiaoHan-Chung Wu
Sep 22, 2016·Journal of Molecular Cell Biology·Yanzhou ZhangSudhakar Jha
Apr 10, 2008·International Journal of Cancer. Journal International Du Cancer·Bernardina T F van der GunPamela M J McLaughlin
Jan 11, 2007·British Journal of Cancer·P A Baeuerle, O Gires
Dec 6, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Laurent DolléLeo A van Grunsven
Feb 13, 2020·Biomolecules·M Aiman MohtarTeck Yew Low
Dec 21, 2020·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Chang M KangKuk H Kwon

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