Methylene blue attenuates renal ischemia-reperfusion injury in rats

Journal of Pediatric Surgery
Fatma SaracIbrahim Aydogdu

Abstract

In our study, we investigated the effects of methylene blue (MB) on histopathological changes in renal ischemia/reperfusion (I/R) injury rat model. Twenty-one Sprague-Dawley male rats were divided equally into three groups. Group 1 (control) was administered intraperitoneal saline solution. In Groups 2 (untreated group) and 3 (MB treatment), the renal arteries were clamped, and ischemia (for 1 hour) and then reperfusion (for 4 hours) were applied. Thirty minutes before ischemia, the untreated group received physiological saline, whereas the treatment group was administered 30 mg/kg MB through an intraperitoneal route. Blood samples were drawn, and renal specimens were harvested 5.5 hours after physiologic saline injection in the control and immediately after the reperfusion period in the other groups. The levels of tissue superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), total oxidant status (TOS), total antioxidant status (TAS), plasma urea, creatinine and ischemia modified albumin (IMA) were measured. Moreover, the histopathological damage score of the renal tissue was determined. MB significantly alleviated the severity of histopathological damage by increasing the levels of tissue SOD and TAS and dec...Continue Reading

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Citations

Oct 17, 2019·International Journal of Molecular Sciences·Ricardo O S SoaresOrlando Castro-E-Silva
Jan 30, 2020·Minerva anestesiologica·Luiz E MirandaPaulo R Barbosa Evora
May 2, 2018·Mediators of Inflammation·Liangrong WangXiangqing Xiong
Jul 1, 2020·Journal of Pediatric and Adolescent Gynecology·Orkun IlgenNedim Karadadas

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