Methylene blue induced morphological deformations in Plasmodium falciparum gametocytes: implications for transmission-blocking

Malaria Journal
Ishan WadiNeena Valecha

Abstract

Malaria remains a global health problem despite availability of effective tools. For malaria elimination, drugs targeting sexual stages of Plasmodium falciparum need to be incorporated in treatment regimen along with schizonticidal drugs to interrupt transmission. Primaquine is recommended as a transmission blocking drug for its effect on mature gametocytes but is not extensively utilized because of associated safety concerns among glucose-6-phosphate dehydrogenase (G6PD) deficient patients. In present work, methylene blue, which is proposed as an alternative to primaquine is investigated for its gametocytocidal activity amongst Indian field isolates. An effort has been made to establish Indian field isolates of P. falciparum as in vitro model for gametocytocidal drugs screening. Plasmodium falciparum isolates were adapted to in vitro culture and induced to gametocyte production by hypoxanthine and culture was enriched for gametocyte stages using N-acetyl-glucosamine. Gametocytes were incubated with methylene blue for 48 h and stage specific gametocytocidal activity was evaluated by microscopic examination. Plasmodium falciparum field isolates RKL-9 and JDP-8 were able to reproducibly produce gametocytes in high yield and were ...Continue Reading

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Citations

Nov 30, 2019·Future Medicinal Chemistry·Ishan WadiAbhinav Sinha
May 8, 2020·Future Medicinal Chemistry·Ishan WadiAbhinav Sinha
May 15, 2020·The Journal of Antimicrobial Chemotherapy·Mathieu GendrotBruno Pradines
Dec 4, 2020·Journal of Ethnopharmacology·Danielle Snider, Pamela J Weathers
Apr 1, 2021·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Luiz Miguel PereiraAna Patrícia Yatsuda
Apr 7, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Isha PandeyAshis Das
May 1, 2021·Pharmaceuticals·Mathieu GendrotOn Behalf Of The French National Reference Centre For Imported Malaria Study Group

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