PMID: 2502945Aug 15, 1989Paper

Methylene blue plus light mediates 8-hydroxyguanine formation in DNA

Archives of Biochemistry and Biophysics
R A FloydJ E Schneider

Abstract

Exposure to methylene blue (MB) plus light mediates formation of large levels of 8-hydroxyguanine in DNA. The amount of 8-hydroxy-2'-deoxyguanosine (8-OHdG) present in DNA increased as the amount of MB concentration increased throughout the 2 to 200 microM range studied and was dependent on light exposure. As the time of light exposure increased so did the 8-OHdG content to levels of about 750 8-OHdG/10(5) deoxyguanosine after 15 min of light exposure when MB was at 20 microM. Even though previous research has demonstrated that hydroxyl free radicals formed from a variety of sources mediate 8-OHdG formation in DNA, inclusion of mannitol, superoxide dismutase, catalase, and desferal in the MB plus light experiments demonstrated that these scavengers of oxygen free radical intermediates or precursors caused either no change or an increase in the 8-OHdG content of DNA exposed to MB plus light. These results appear to rule out the direct role of oxygen free radical intermediates in the primary events involved in the MB plus light mediated formation of 8-OHdG in DNA. Oxygen was essential to cause MB plus light mediated 8-OHdG formation in DNA. It was noted that when the reaction was carried out where the deuterium oxide content had ...Continue Reading

References

Jan 1, 1988·Free Radical Biology & Medicine·J E SchneiderR A Floyd
Feb 27, 1973·Biochemistry·H J FormanI Fridovich

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Citations

Oct 1, 1995·Journal of Medical Virology·B BachmannH Mohr
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