Methylene chloride fraction of Scutellaria barbata induces apoptosis in human U937 leukemia cells via the mitochondrial signaling pathway

Clinica Chimica Acta; International Journal of Clinical Chemistry
Yun-Yi ChaSung-Hoon Kim

Abstract

Scutellaria barbata D.Don has been applied to treat cancers, inflammation and urinary disease. However, its antitumor mechanism still remains unclear. With methylene chloride fraction of Herba Scutellariae barbatae (MCSB), apoptosis-related experiments were carried out on human U937 leukemia cells by (a) 2,3-bis[2-4-nitro-5-sulphophenyl]2H-tetrazolium-5-carboxanilide (XTT) assay for cytotoxicity; (b) terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling (TUNEL) assay for morphological changes; (c) cell cycle analysis; (d) Western blot analysis of poly(ADP-ribose) polymerase (PARP), caspase-8, caspase-9, caspase-3 and Bax, Bcl-2 and cytochrome c expressions for apoptosis signaling pathway. MCSB inhibited the proliferation of human U937 leukemia cells in a dose-dependent manner (IC50 = approximately 10 microg/ml). MCSB dose-dependently increased the sub-G1 DNA contents by cell cycle analysis. DNA fragments indicating induction of apoptosis were observed in MCSB-treated U937 cells by TUNEL assay. Caspase-9 and caspase-3 were activated while caspase-8 was intact by MCSB. Similarly, MCSB effectively cleaved PARP, increased the ratio of Bax/Bcl-2 and released the cytochrome c from mitochondria during apoptosis in U937...Continue Reading

References

Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y A LazebnikW C Earnshaw
Jul 1, 1993·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·B Y WongR W Teel
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D L Vaux
Mar 4, 1998·Biochemical Pharmacology·R G Panchal
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J M JürgensmeierJ C Reed
Feb 1, 1996·Cancer Biotherapy & Radiopharmaceuticals·B Y WongC P Wan
May 11, 2002·Nature Reviews. Cancer·Frederik H Igney, Peter H Krammer
Nov 8, 2003·Journal of Asian Natural Products Research·Jin-Lan ZhangTong-Hui Zhou
Jan 22, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jin-Mu YiHyung-Min Kim

❮ Previous
Next ❯

Citations

Dec 1, 2009·Cancer Cell International·Gowhar ShafiDavid K Y Lei
Nov 18, 2006·Journal of Zhejiang University. Science. B·Wen-jing RuanJian-guang Zhou
Dec 26, 2008·World Journal of Gastroenterology : WJG·Zhi-Jun DaiLing-Qin Song
Mar 29, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Shih-Chin WangJung-Yaw Lin
Jan 13, 2010·Journal of Ethnopharmacology·Xiaofei ShangZhengping Jia
Nov 30, 2012·Phytotherapy Research : PTR·Haitao Xu, Songyan Zhang
Apr 11, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Fon-Chang LiuChieh-Hsi Wu
Dec 24, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Zahra Tayarani-NajaranSeyed Hadi Mousavi
Nov 6, 2012·Ageing Research Reviews·Venus SosaMatilde E LLeonart
May 31, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhi-Jun DaiXi-Jing Wang
May 16, 2009·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Brian Y Y WongJianping Zheng
Aug 4, 2007·Phytotherapy Research : PTR·Jianqing YuGuolin Zou
Jul 30, 2019·Frontiers in Pharmacology·Weixiao AnShousong Cao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.