PMID: 9428714Jan 15, 1998Paper

Methylmalonyl-CoA decarboxylase from Propionigenium modestum--cloning and sequencing of the structural genes and purification of the enzyme complex

European Journal of Biochemistry
M BottP Dimroth

Abstract

Methylmalonyl-CoA decarboxylase catalyses the only energy-conserving step during succinate fermentation by Propionigenium modestum: the decarboxylation of (S)-methylmalonyl-CoA to propionyl-CoA is coupled to the vectorial transport of Na+ across the cytoplasmic membrane, thereby creating a sodium ion motive force that is used for ATP synthesis. By taking advantage of the sequence similarity between the beta-subunits of other Na+-transport decarboxylases, a portion of the P. modestum beta-subunit gene was amplified by PCR with degenerated primers. The cloned PCR product then served as homologous probe for cloning suitable fragments from genomic DNA. Sequence analysis of a 3.7-kb region identified four genes which probably form a transcriptional unit, mmdADCB. Remarkably, a mmdE gene which is present in the homologous mmdADECB cluster from Veillonella parvula and encodes the 6-kDa epsilon-subunit, is missing in P. modestum. By sequence comparisons, the following functions could be assigned to the P. modestum proteins: MmdA (56.1 kDa; alpha-subunit), carboxyltransferase; MmdB (41.2 kDa; beta-subunit), carboxybiotin-carrier-protein decarboxylase; MmdC (13.1 kDa; gamma-subunit), biotin carrier protein. MmdD (14.2 kDa; delta-subunit)...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
May 1, 1992·Journal of General Microbiology·K Denger, B Schink
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Jan 16, 1997·Biochimica Et Biophysica Acta·P Dimroth

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Citations

Jan 21, 2003·Journal of Bacteriology·Songkran ChuakrutYasuo Igarashi
Aug 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·C C HäseP A Dibrov
Apr 29, 2006·Journal of Molecular Microbiology and Biotechnology·Clara D BoiangiuWolfgang Buckel
Mar 25, 2014·PloS One·Martin J SergeantMark J Pallen
Feb 22, 2000·The Journal of Biological Chemistry·A L McKeanE S Wurtele
Jan 14, 2021·Microbial Biotechnology·Pauline L FolchRuud A Weusthuis
Jul 31, 1999·Applied and Environmental Microbiology·O KniemeyerJ Harder
Mar 15, 2001·Biochimica Et Biophysica Acta·W Buckel
Jul 30, 2021·Biotechnology and Applied Biochemistry·S Balaji

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