Methylmercury exposure causes a persistent inhibition of myogenin expression and C2C12 myoblast differentiation

Toxicology
Lisa M Prince, M D Rand

Abstract

Methylmercury (MeHg) is a ubiquitous environmental toxicant, best known for its selective targeting of the developing nervous system. MeHg exposure has been shown to cause motor deficits such as impaired gait and coordination, muscle weakness, and muscle atrophy, which have been associated with disruption of motor neurons. However, recent studies have suggested that muscle may also be a target of MeHg toxicity, both in the context of developmental myogenic events and of low-level chronic exposures affecting muscle wasting in aging. We therefore investigated the effects of MeHg on myotube formation, using the C2C12 mouse myoblast model. We found that MeHg inhibits both differentiation and fusion, in a concentration-dependent manner. Furthermore, MeHg specifically and persistently inhibits myogenin (MyoG), a transcription factor involved in myocyte differentiation, within the first six hours of exposure. MeHg-induced reduction in MyoG expression is contemporaneous with a reduction of a number of factors involved in mitochondrial biogenesis and mtDNA transcription and translation, which may implicate a role for mitochondria in mediating MeHg-induced change in the differentiation program. Unexpectedly, inhibition of myoblast differ...Continue Reading

References

Oct 1, 1983·American Journal of Epidemiology·G E McKeown-EyssenA Neims
Jul 1, 1982·Experimental Neurology·P R SagerP M Rodier
Dec 11, 1997·Neurotoxicology and Teratology·P GrandjeanP J Jørgensen
Jan 23, 1998·Toxicologic Pathology·K Eto
Mar 6, 1999·The Journal of Biological Chemistry·K KurodaT Honjo
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 8, 2002·Environmental Research·Sylvaine CordierClaudine Amiel-Tison
Feb 15, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Philippe GrandjeanPál Weihe
Apr 16, 2003·Differentiation; Research in Biological Diversity·Bret B FridayGrace K Pavlath
Dec 4, 2003·Science·Irina M ConboyThomas A Rando
Sep 8, 2004·Brain Research. Developmental Brain Research·Mineshi SakamotoHitoshi Takahashi
Nov 2, 2005·Journal of Cellular Physiology·Pascal SeyerChantal Wrutniak-Cabello
Feb 21, 2006·Neurotoxicology and Teratology·Cindy S Roegge, Susan L Schantz
Jun 8, 2006·Neurotoxicology·Christin Bland, Matthew D Rand
Mar 6, 2007·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M AschnerM Farina
Feb 28, 2008·Neuroreport·Christoffer TammSandra Ceccatelli
Mar 28, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Matthew D RandJeffrey Bond
Aug 30, 2008·Journal of Cellular Physiology·Matthew F BuasTom Kadesch
Oct 30, 2008·Journal of Cell Science·Danqiong SunAnna Zolkiewska
Mar 3, 2010·Environmental Health Perspectives·Bo-Eun LeeEun-Hee Ha
Mar 20, 2010·Environmental Health Perspectives·Yuan-Peng YenShing-Hwa Liu
Nov 27, 2010·Molecular and Cellular Biochemistry·Akira WagatsumaShigeru Yamada
Jun 2, 2011·The Journal of Toxicological Sciences·Nobuko MoriKimiko Hirayama
May 1, 2008·Environmental Toxicology and Pharmacology·Ciro Alberto de Oliveira RibeiroJean Charles Massabuau
Oct 18, 2011·The Science of the Total Environment·Byung-Mi KimEun-Hee Ha
Nov 3, 2011·Stem Cells·Christopher R R BjornsonThomas A Rando
Jan 11, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G L EngelM D Rand
Jan 26, 2012·Environmental Health Perspectives·Margaret R KaragasSusan Korrick
Jul 20, 2012·Comparative and Functional Genomics·Herve Faralli, F Jeffrey Dilworth
Aug 29, 2012·Journal of Biomedicine & Biotechnology·Daniel RoosNilda Vargas Barbosa

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Citations

Jul 24, 2018·Drug and Chemical Toxicology·Thaís Doeler AlgarveIvana Beatrice Mânica da Cruz
Dec 19, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Samuel W CaitoMichael Aschner
Aug 18, 2020·Toxicology·Ashley E PeppriellMatthew D Rand
Sep 22, 2021·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jakob T GundersonMatthew D Rand

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