PMID: 16501822Feb 28, 2006Paper

Methylmercury intoxication activates nitric oxide synthase in chick retinal cell culture

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
Anderson Manoel HerculanoJ L M do Nascimento

Abstract

The visual system is a potential target for methylmercury (MeHg) intoxication. Nevertheless, there are few studies about the cellular mechanisms of toxicity induced by MeHg in retinal cells. Various reports have indicated a critical role for nitric oxide synthase (NOS) activation in modulating MeHg neurotoxicity in cerebellar and cortical regions. The aim of the present study is to describe the effects of MeHg on cell viability and NOS activation in chick retinal cell cultures. For this purpose, primary cultures were prepared from 7-day-old chick embryos: retinas were aseptically dissected and dissociated and cells were grown at 37 degrees C for 7-8 days. Cultures were exposed to MeHg (10 microM, 100 microM, and 1 mM) for 2, 4, and 6 h. Cell viability was measured by MTT method and NOS activity by monitoring the conversion of L-[H3]-arginine to L-[H3]-citrulline. The incubation of cultured retina cells with 10 and 100 microM MeHg promoted an increase of NOS activity compared to control (P < 0.05). Maximum values (P < 0.05) were reached after 4 h of MeHg incubation: increases of 81.6 +/- 5.3 and 91.3 +/- 3.7%, respectively (data are reported as mean +/- SEM for 4 replicates). MeHg also promoted a concentration- and time-dependen...Continue Reading

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Citations

Apr 25, 2012·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Bingzhen CaoXia Cao
Oct 31, 2008·Environmental Health : a Global Access Science Source·Jean-Paul BourdineaudMuriel Laclau
Jan 25, 2012·International Journal of Molecular Sciences·Preeyaporn Koedrith, Young Rok Seo
May 24, 2011·Toxicology and Applied Pharmacology·Marcelo FarinaJoão B T Rocha
Sep 16, 2009·Neurochemistry International·Karen Renata Matos OliveiraJosé Luiz Martins do Nascimento
Sep 15, 2009·Acta histochemica·Cristiane do Socorro Ferraz MaiaCristovam Wanderley Picanço Diniz
May 8, 2007·Neuroscience Research·Ricardo Augusto de Melo ReisJosé Luis Martins do Nascimento
Feb 13, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Samuel W Caito, Michael Aschner
Oct 21, 2014·PloS One·Allan Costa-MalaquiasMaría Elena Crespo-Lopez
Jun 25, 2015·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·L A L MauésJ L M do Nascimento
Sep 30, 2006·Environment International·María Elena Crespo-LópezJosé Luiz Martins do Nascimento
May 20, 2015·Archives of Environmental Contamination and Toxicology·Felipe André dos SantosPedro de Magalhães Padilha
Jul 1, 2012·Toxicology Research·Stephanie Jb FrethamMichael Aschner
Mar 17, 2019·Journal of Molecular Neuroscience : MN·Geir BjørklundJan Aaseth
Jul 23, 2021·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Priscila Cunha NascimentoRafael Rodrigues Lima
Oct 2, 2007·Environmental Research·M C N PinheiroJ L M do Nascimento

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