PMID: 9436471Jun 1, 1997Paper

Methylprednisolone and 2-chloroadenosine induce DNA fragmentation at different stages of human T-lymphocyte development

Immunology Letters
Z Szondy

Abstract

Both methylprednisolone (MPS) and 2-chloroadenosine (2-CA) were shown previously to induce DNA fragmentation and cell death in human thymocytes at an optimum concentration of 1 and 40 microM, respectively. Though both compounds affected the CD4+CD8+ population, 2-CA depleted primarily thymocytes expressing medium or high levels of CD3-T-cell receptor molecule, while the glucocorticoid treatment affected cells expressing a lower level of CD3-T-cell receptor. Their effect on thymocyte viability and DNA fragmentation was observed already at day 1 of culture and involved the bcl-2 negative thymocytes. Incubation of peripheral T-lymphocytes (which express bcl-2) with the same concentration of MPS did not affect the viability for up to 5 days, while 2-CA induced 100% cell death and DNA fragmentation by day 5. If T-cells were stimulated with concanavalin A in the presence of MPS or 2-CA the cell proliferation was inhibited and a decrease in cell viability with a concomittant increase in DNA fragmentation was observed. If MPS was added at 24 h or later after mitogenic stimulation, it was not able to induce apoptosis and the inhibition of proliferation was less pronounced. 2-CA, on the other hand, inhibited proliferation and induced cel...Continue Reading

References

Aug 1, 1990·Immunology Today·F Di VirgilioD Collavo
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Dec 1, 1993·Immunology Today·M Groettrup, H von Boehmer

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Citations

Mar 19, 2013·European Journal of Orthopaedic Surgery & Traumatology : Orthopédie Traumatologie·Irfan GungorKadir Kaya
Feb 5, 2008·Biochemical Pharmacology·Laurent Bastin-CoyetteFrançoise Bontemps
Jun 30, 2004·Cell Biology International·Ridvan YalcinErkan Yurtcu
Feb 24, 2001·Annals of the New York Academy of Sciences·L B Chiarini, R Linden

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