Methylthioadenosine and polyamine biosynthesis in a Saccharomyces cerevisiae meu1delta mutant

Biochemical and Biophysical Research Communications
Manas K ChattopadhyayHerbert Tabor

Abstract

As part of our studies on polyamine biosynthesis in yeast, the metabolism of methylthioadenosine was studied in a mutant that lacks methylthioadenosine phosphorylase (meu1delta). The nucleoside accumulates in this mutant and is mainly excreted into the culture medium. Intracellular accumulation of the nucleoside is enough to account for the inhibition of spermidine synthase and thus to indirectly regulate the polyamine content of the meu1delta cells. By comparing the results with this mutant with a meu1delta spe2delta mutant that cannot synthesize spermidine or spermine, we showed that >98% of methylthioadenosine is produced as a byproduct of polyamine synthesis (i.e., from decarboxylated S-adenosylmethionine). In contrast, in MEU1+ SPE2+ cells methylthioadenosine does not accumulate and is metabolized through the methionine salvage pathway. Using a met15delta mutant we show that this pathway (i.e., involving polyamine biosynthesis and methylthioadenosine metabolism) is a significant factor in the metabolism of methionine, accounting for 15% of the added methionine.

References

Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D BalasundaramH Tabor
Sep 19, 1986·Biochimica Et Biophysica Acta·H YamanakaD A Carson
Sep 1, 1985·Journal of General Microbiology·K S Marchitto, A J Ferro
Jan 1, 1983·Methods in Enzymology·A E Pegg
Feb 1, 1982·Biochemical Pharmacology·H G Williams-AshmanP Galletti
Apr 15, 1982·Archives of Biochemistry and Biophysics·I H FoxC Herring
Dec 31, 1981·Biochemical and Biophysical Research Communications·P C Trackman, R H Abeles
May 1, 1980·The Biochemical Journal·H HibasamiA E Pegg
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·T NoboriD A Carson
Oct 3, 2000·The Journal of Biological Chemistry·D ThomasY Surdin-Kerjan
Sep 25, 2003·The Journal of Biological Chemistry·Ahmad L SubhiWarren D Kruger
Nov 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Manas K ChattopadhyayHerbert Tabor

❮ Previous
Next ❯

Citations

Apr 12, 2007·Proceedings of the National Academy of Sciences of the United States of America·Michael G MalkowskiElizabeth J Grayhack
Jul 3, 2008·Planta·T KusanoY Takahashi
Jul 20, 2014·Journal of Biomedical Science·He-Yen ChouLi-Mei Pai
Aug 14, 2019·Nature Communications·Prashanth Srinivasan, Christina D Smolke
Sep 6, 2007·Neoplasia : an International Journal for Oncology Research·Shane W OramZhou Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.