Methyltransferase-like 1 (METTL1) served as a tumor suppressor in colon cancer by activating 7-methyguanosine (m7G) regulated let-7e miRNA/HMGA2 axis

Life Sciences
Yang LiuBoshi Sun

Abstract

This study aimed to uncover the underlying mechanisms of METTL1/let-7e miRNA/HMGA2 axis regulated colon cancer (CC) development. Real-Time qPCR was used to detect the expression levels of METTL1 mRNA and let-7e miRNA in clinical specimens and cell lines. Pearson correlation analysis was conducted to analyze the correlation of let-7e miRNA and METTL1 mRNA in cancer tissues. Western Blot was performed to examine protein expressions. Cell proliferation was evaluated by CCK-8 assay and cell mobility was determined by transwell assay. Dual-luciferase reporter gene system was used to validate the binding sites of let-7e miRNA and 3' UTR regions of HMGA2 mRNA. METTL1 and let-7e miRNA were low-expressed in CC tissues and cells compared to their normal counterparts. Overexpression of METTL1 inhibited CC cell proliferation, invasion as well as migration, and promoted cell apoptosis. Further results validated that METTL1 overexpression increased the levels of let-7e miRNA in CC cell lines, and the effects of overexpressed METTL1 on the above cell functions were reversed by knocking down let-7e miRNA. In addition, high mobility group AT-hook 2 (HMGA2) was the downstream target of let-7e miRNA, and overexpressed METTL1 inhibited HMGA2 expre...Continue Reading

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Citations

Jun 7, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
Jul 1, 2021·Molecular Therapy. Nucleic Acids·Dawei RongXuehao Wang
Aug 6, 2021·Molecular Cell·Esteban A OrellanaRichard I Gregory

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