PMID: 6162526Mar 2, 1981Paper

Methylxanthines modulate adenosine release from slices of cerebral cortex

Brain Research
T W StoneH G Lloyd

Abstract

Using slices of mouse or rat cerebral cortex incubated with [3H]adenosine or [3H]adenine and/or [14C]GABA we have examined factors affecting the release of these compounds, and especially the influence of methylxanthines. Although release of purines and GABA could be induced by ouabain (10(-4) M), or p-hydroxymercuribenzoate (5 x 10(-4) M) no release was produced by ethacrynic acid (10(-3) or 10(-4) M) phenytoin (10(-3) M), noradrenaline or SC 13504. Release is probably not therefore related to (Na+, K+) ATPase or Mg2+-ATPase inhibition. At concentrations of 10(-3) and 10(-4) M, caffeine, theophylline, aminophylline and isobutyl-methylxanthine (IBMX) markedly depressed the release of purines evoked by ouabain. Non-xanthine inhibition of phosphodiesterase had much weaker though statistically significant effects. The methylxanthines had no significant effect on GABA release. It is suggested that the results can be explained on the basis of a positive feedback system in which released adenosine activates membranal adenylate cyclase, and the increased concentration of cyclic AMP which results form or origin of much of the adenosine released subsequently. However, we cannot exclude the existence of an intracellular receptor for meth...Continue Reading

References

Jan 1, 1978·Biochemical Society Transactions·T W Stone
Sep 21, 1979·Journal of Theoretical Biology·J A Ribeiro
Feb 1, 1976·Pharmacological Research Communications·H D Mah, J W Daly
May 1, 1976·European Journal of Pharmacology·T KarasawaM Shimizu
Jul 1, 1971·The Journal of Physiology·W D PatonM A Zar
Apr 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·H ShimizuJ Daly
Nov 1, 1971·The Journal of Pharmacy and Pharmacology·B Waldeck
Jun 1, 1980·The Journal of Physiology·C Hollins, T W Stone
Dec 1, 1963·Canadian Journal of Biochemistry and Physiology·R I BIRKS
Aug 1, 1976·Neurochemical Research·H McIlwain

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Citations

Apr 15, 1994·Pharmacy World & Science : PWS·M J HigginsT W Stone
Mar 7, 1983·Life Sciences·J P BoulengerP J Marangos
Jan 1, 1988·Progress in Biophysics and Molecular Biology·H C Hartzell
May 18, 1984·Neuroscience Letters·R A BarracoR F Berman
Jan 1, 1985·General Pharmacology·R A BarracoH J Altman

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