Mar 24, 2010

Metnase/SETMAR: a domesticated primate transposase that enhances DNA repair, replication, and decatenation

Genetica
Montaser ShaheenRobert Hromas

Abstract

Metnase is a fusion gene comprising a SET histone methyl transferase domain and a transposase domain derived from the Mariner transposase. This fusion gene appeared first in anthropoid primates. Because of its biochemical activities, both histone (protein) methylase and endonuclease, we termed the protein Metnase (also called SETMAR). Metnase methylates histone H3 lysine 36 (H3K36), improves the integration of foreign DNA, and enhances DNA double-strand break (DSB) repair by the non-homologous end joining (NHEJ) pathway, potentially dependent on its interaction with DNA Ligase IV. Metnase interacts with PCNA and enhances replication fork restart after stalling. Metnase also interacts with and stimulates TopoIIalpha-dependent chromosome decatenation and regulates cellular sensitivity to topoisomerase inhibitors used as cancer chemotherapeutics. Metnase has DNA nicking and endonuclease activity that linearizes but does not degrade supercoiled plasmids. Metnase has many but not all of the properties of a transposase, including Terminal Inverted Repeat (TIR) sequence-specific DNA binding, DNA looping, paired end complex formation, and cleavage of the 5' end of a TIR, but it cannot efficiently complete transposition reactions. Inter...Continue Reading

  • References33
  • Citations30

References

  • References33
  • Citations30

Citations

Mentioned in this Paper

Fusion Gene
Non-Homologous DNA End-Joining
Biochemical Pathway
Antineoplastic Agents
Histone antigen
Transposase
DNA ligase IV
Transposase Activity
DNA Repair
Endonuclease

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