Metrics of separation performance in chromatography: Part 3: General separation performance of linear solvent strength gradient liquid chromatography

Journal of Chromatography. a
L M Blumberg, Gert Desmet

Abstract

The separation performance metrics defined in Part 1 of this series are applied to the evaluation of general separation performance of linear solvent strength (LSS) gradient LC. Among the evaluated metrics was the peak capacity of an arbitrary segment of a chromatogram. Also evaluated were the peak width, the separability of two solutes, the utilization of separability, and the speed of analysis-all at an arbitrary point of a chromatogram. The means are provided to express all these metrics as functions of an arbitrary time during LC analysis, as functions of an arbitrary outlet solvent strength changing during the analysis, as functions of parameters of the solutes eluting during the analysis, and as functions of several other factors. The separation performance of gradient LC is compared with the separation performance of temperature-programmed GC evaluated in Part 2.

References

Jun 14, 2001·Journal of Chromatography. a·L M Blumberg, M S Klee
Jan 5, 2002·Journal of Chromatography. a·L M Blumberg, M S Kle
Jul 26, 2005·Journal of Chromatography. a·Uwe D Neue
Feb 14, 2006·Journal of Chromatography. a·Uwe D NeueLloyd R Snyder
Jan 1, 1944·The Biochemical Journal·R ConsdenA J Martin
Feb 7, 2007·Journal of Chromatography. a·Fabrice Gritti, Georges Guiochon
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Jan 1, 2008·Journal of Chromatography. a·Uwe Dieter Neue
Oct 28, 2008·Journal of Chromatography. a·Fabrice Gritti, Georges Guiochon

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Citations

Jan 13, 2016·Analytical Chemistry·Leonid M Blumberg, Gert Desmet
Jun 4, 2019·Journal of Chromatography. a·Leonid M Blumberg

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