METTL13 is downregulated in bladder carcinoma and suppresses cell proliferation, migration and invasion.

Scientific Reports
Zhe ZhangXiaojun Man

Abstract

The incidence of bladder cancer has increased in the last few decades, thus novel markers for early diagnosis and more efficacious treatment are urgently needed. It found that METTTL13 protein is aberrant expression in variety of human cancers and METTL13 was involved in oncogenic pathways. However, the role of METTL13 has been unexplored in bladder cancer to date. Here, expression of METTL13 was lower in bladder cancer tissue samples and cancer cell lines than in normal bladder tissue and cell lines. METTL13 was downregulated in the late stages of the disease and was maintained at low level throughout the tumor progression process based on tumor node metastasis (TNM) staging. Further research suggested that METTL13 negatively regulates cell proliferation in bladder cancer and reinstates G1/S checkpoint via the coordinated downregulation of CDK6, CDK4 and CCND1, decreased phosphorylation of Rb and subsequent delayed cell cycle progression. Moreover, METTL13-dependent inhibition of bladder cancer cell migration and invasion is mediated by downregulation of FAK (Focal adhesion kinase) phosphorylation, AKT (v-akt murine thymoma viral oncogene) phosphorylation, β-catenin expression and MMP-9 expression. These integrated efforts hav...Continue Reading

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Citations

Sep 1, 2016·Scientific Reports·Wen-Bo WuHai-Tao Liu
Sep 24, 2016·Journal of Translational Medicine·Yan LiAtsushi Takahashi
May 19, 2018·Nature Reviews. Drug Discovery·P Ann Boriack-SjodinRobert A Copeland
Apr 5, 2018·GigaScience·Shuai JiangJian Ren
Dec 27, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Chirag NepalJesper B Andersen
Sep 5, 2018·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yan LiAtsushi Takahashi
Nov 28, 2018·Chembiochem : a European Journal of Chemical Biology·Rong Huang
Jul 23, 2021·Insect Molecular Biology·L BatagliaF M F Nunes

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Methods Mentioned

BETA
dissection
PCR
protein assay
transfection
light microscopy
transgenic
xenograft

Software Mentioned

SDS
ImageJ
Cell Quest
SPSS ( Statistical Package for the Social Sciences )

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