Mex3c regulates insulin-like growth factor 1 (IGF1) expression and promotes postnatal growth.

Molecular Biology of the Cell
Yan JiaoBaisong Lu

Abstract

Insulin-like growth factor 1 (IGF1) mediates the growth-promoting activities of growth hormone. How Igf1 expression is regulated posttranscriptionally is unclear. Caenorhabditis elegans muscle excess 3 (MEX-3) is involved in cell fate specification during early embryonic development through regulating mRNAs involved in specifying cell fate. The function of its mammalian homologue, MEX3C, is unknown. Here we show that MEX3C deficiency in Mex3c homozygous mutant mice causes postnatal growth retardation and background-dependent perinatal lethality. Hypertrophy of chondrocytes in growth plates is significantly impaired. Circulating and bone local production of IGF1 are both decreased in mutant mice. Mex3c mRNA is strongly expressed in the testis and the brain, and highly expressed in resting and proliferating chondrocytes of the growth plates. MEX3C is able to enrich multiple mRNA species from tissue lysates, including Igf1. Igf1 expression in bone is decreased at the protein level but not at the mRNA level, indicating translational/posttranslational regulation. We propose that MEX3C protein plays an important role in enhancing the translation of Igf1 mRNA, which explains the perinatal lethality and growth retardation observed in M...Continue Reading

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Citations

Aug 29, 2012·Molecular and Cellular Biology·Yan JiaoBaisong Lu
Apr 18, 2014·The Journal of Endocrinology·Changjie HanBaisong Lu
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Kanako KuniyoshiTaro Kawai
Jul 29, 2020·International Journal of Molecular Sciences·Simon Jasinski-BergnerBarbara Seliger
Oct 23, 2018·Frontiers in Genetics·Tássia Mangetti GonçalvesLuiz Lehmann Coutinho
Apr 3, 2019·International Journal of Molecular Sciences·Lee Lee WongPeipei Wang
Mar 9, 2021·JCI Insight·David W DodingtonMinna Woo
Jun 3, 2021·Biology·Marcell LedererStefan Hüttelmaier

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Datasets Mentioned

BETA
BR000945
CJ072395.1
BC125427

Methods Mentioned

BETA
gene trap
gene trapping
enzyme-linked immunosorbent assay
ELISA
pull-down
pulled down
pull down
biopsies
genotyping
gene

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