MF59 and Pam3CSK4 boost adaptive responses to influenza subunit vaccine through an IFN type I-independent mechanism of action

The Journal of Immunology : Official Journal of the American Association of Immunologists
Elena CaproniEnnio De Gregorio

Abstract

The innate immune pathways induced by adjuvants required to increase adaptive responses to influenza subunit vaccines are not well characterized. We profiled different TLR-independent (MF59 and alum) and TLR-dependent (CpG, resiquimod, and Pam3CSK4) adjuvants for the ability to increase the immunogenicity to a trivalent influenza seasonal subunit vaccine and to tetanus toxoid (TT) in mouse. Although all adjuvants boosted the Ab responses to TT, only MF59 and Pam3CSK4 were able to enhance hemagglutinin Ab responses. To identify innate immune correlates of adjuvanticity to influenza subunit vaccine, we investigated the gene signatures induced by each adjuvant in vitro in splenocytes and in vivo in muscle and lymph nodes using DNA microarrays. We found that flu adjuvanticity correlates with the upregulation of proinflammatory genes and other genes involved in leukocyte transendothelial migration at the vaccine injection site. Confocal and FACS analysis confirmed that MF59 and Pam3CSK4 were the strongest inducers of blood cell recruitment in the muscle compared with the other adjuvants tested. Even though it has been proposed that IFN type I is required for adjuvanticity to influenza vaccines, we found that MF59 and Pam3CSK4 were n...Continue Reading

References

Mar 10, 1977·The New England Journal of Medicine·P A Gross, F A Ennis
Jun 25, 1998·Cellular Immunology·M DupuisD M McDonald
Oct 10, 2001·European Journal of Immunology·M DupuisG Ott
Jun 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Enrico ProiettiFilippo Belardelli
Dec 14, 2002·Human Immunology·Norimitsu Kadowaki, Yong-Jun Liu
Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·John D Storey, Robert Tibshirani
Oct 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ulrike Wille-ReeceRobert A Seder
Feb 12, 2008·The Journal of Infectious Diseases·David I BernsteinHeather Hill
Apr 9, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anja SeubertAndreas Wack
Jul 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·F MoscaE De Gregorio
Sep 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mirjam KoolJürg Tschopp
Feb 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Grazia GalliFlora Castellino
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Grazia GalliIain Stephenson
Jun 30, 2009·The Pediatric Infectious Disease Journal·Timo VesikariAudino Podda
Sep 12, 2009·The New England Journal of Medicine·Tristan W ClarkIain Stephenson
Apr 29, 2010·Science Translational Medicine·Shohei KoyamaKen J Ishii

❮ Previous
Next ❯

Citations

Jun 4, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Daniel O'Connor, Andrew J Pollard
Dec 7, 2013·Nature Medicine·Steven G ReedChristopher B Fox
Jul 25, 2014·Journal of Immunology Research·Afonso P Basto, Alexandre Leitão
Dec 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Maria VonoAnja Seubert
Mar 19, 2013·The Journal of Biological Chemistry·Prexy ShahJonathan P Butchar
Jul 5, 2013·Clinical Microbiology Reviews·Sook-San Wong, Richard J Webby
Nov 30, 2012·Expert Opinion on Biological Therapy·Giuseppe Del GiudiceRino Rappuoli
May 30, 2015·Human Vaccines & Immunotherapeutics·Melissa SantoneUgo D'Oro
Dec 14, 2016·Scientific Reports·Thorunn A OlafsdottirAli M Harandi
Feb 13, 2016·EBioMedicine·Karen A O MartinsSina Bavari
Jan 13, 2018·Frontiers in Immunology·Amanda L WilkinsAndrew J Pollard
Jul 18, 2018·Human Vaccines & Immunotherapeutics·Eun-Ju Ko, Sang-Moo Kang
Apr 23, 2019·Expert Review of Vaccines·Indranil SarkarSylvia van Drunen Littel-van den Hurk
Jul 28, 2013·Expert Review of Vaccines·John P Vasilakos, Mark A Tomai
Aug 6, 2013·Journal of Pharmacokinetics and Pharmacodynamics·Million A Tegenge, Robert J Mitkus
Apr 3, 2020·Immunotherapy·Lizdany Flórez-ÁlvarezJuan C Hernandez
Jan 6, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laura C WhitmoreJessica G Moreland
Mar 25, 2017·Frontiers in Immunology·Marisol Pérez-ToledoConstantino López-Macías
Oct 24, 2018·Veterinary Research·Stina HellmanCaroline Fossum
Aug 17, 2019·Vaccines·Mansi SharmaShashank Tripathi
Nov 27, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Melissa A KachuraJohn D Campbell
Jun 29, 2016·Clinical & Translational Immunology·Jennifer K Dowling, Ashley Mansell
Sep 29, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhaoxia HuangBeiyi Liu
Dec 2, 2020·Seminars in Immunology·Derek T O'HaganGiuseppe Lofano

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

B cell Activation

B cell activation is initiated by the ligation of the B cell receptor with antigen and ultimately results in the production of protective antibodies against potentially pathogenic invaders. Here is the latest research.

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.