PMID: 9418040Jan 7, 1998Paper

Mg-chelatase of tobacco: identification of a Chl D cDNA sequence encoding a third subunit, analysis of the interaction of the three subunits with the yeast two-hybrid system, and reconstitution of the enzyme activity by co-expression of recombinant CHL D, CHL H and CHL I

The Plant Journal : for Cell and Molecular Biology
J PapenbrockBernhard Grimm

Abstract

Mg-protoporphyrin IX chelatase catalyzes insertion of the magnesium ion into protoporphyrin IX, the last common intermediate precursor in chlorophyll and heme biosynthesis, to form Mg-protoporphyrin IX. In Rhodobacter sphaeroides, and Synechocystis, the three open reading frames bchD/chID, bchH/chIH and bchI/chII encode proteins which are required for in vitro Mg-chelatase activity. In higher plants also, three proteins are necessary for the Mg chelation, and genes homologous to bchH and bchI have been isolated previously. In this study, a novel tobacco cDNA sequence homologous to bchD is isolated and initially characterized. Together with the tobacco clones encoding the other two subunits, full-length cDNAs are now available for the first time for all three subunits of one plant species. The CHL D polypeptide deduced from the open reading frame encodes a protein of 758 aa (82.9 kDa) with an amino terminal extension that resembles a plastid transit peptide. Sequence comparison of tobacco CHL D revealed similarities to the D subunit of Rhodobacter and Synechocystis of 44% and 75%. The amino terminal half of CHL D shows significant similarity (46%) to the entire CHL I peptide sequence, indicating a gene duplication from an ancest...Continue Reading

Citations

Sep 3, 2008·Science in China. Series C, Life Sciences·Y HuJ Li
Dec 18, 2004·Plant Molecular Biology·Ulrich EckhardtStefan Hörtensteiner
Jun 15, 2007·Photosynthesis Research·Maryse A BlockNorbert Rolland
Mar 6, 1999·Current Opinion in Plant Biology·B Grimm
May 22, 2004·European Journal of Biochemistry·Vanessa LakeMats Hansson
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·A HanssonM Hansson
Oct 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·A HanssonM Hansson
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·S GräfeF Hänel
Sep 1, 2005·The FEBS Journal·Mark ShepherdC Neil Hunter
Oct 11, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Koichi KobayashiTatsuru Masuda
Jul 15, 2005·Molecular Biology and Evolution·Tuo ShiPaul G Falkowski
May 3, 2007·The Journal of Biological Chemistry·Akinori IkegamiTatsuru Masuda
Feb 12, 2008·The Journal of Biological Chemistry·Nickolche SirijovskiMats Hansson
Jun 6, 2019·The Biochemical Journal·David A FarmerNathan B P Adams
May 30, 2003·Plant & Cell Physiology·Ki-Hong JungGynheung An
Sep 13, 2003·The Plant Journal : for Cell and Molecular Biology·Keara A FranklinMatthew J Terry
Sep 16, 2008·The Journal of Biological Chemistry·Artur Sawicki, Robert D Willows
Mar 31, 2004·The Journal of Biological Chemistry·James D Reid, C Neil Hunter
Aug 8, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Shabnam Tarahi TabriziRobert D Willows
Sep 29, 2017·Frontiers in Plant Science·María I Chacón-Sánchez, Jaime Martínez-Castillo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.