Mg deficiency changes the isoenzyme pattern of reactive oxygen species-related enzymes and regulates NADPH-oxidase-mediated ROS signaling in cotton

Turkish Journal of Biology = Türk Biyoloji Dergisi
Rengin Özgür UzildayI Turkan

Abstract

The aim of this work was to investigate changes in isoenzyme patterns of enzymes related to reactive oxygen species (ROS) detoxification such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR), and glutathione-S-transferase (GST) in cotton under Mg deficiency. Moreover, we aimed to elucidate how a ROS producer, NADPH oxidase (NOX), responds to changing Mg levels. Cotton plants were grown with different concentrations of MgSO 4 in hydroponic medium to create nutrient deficiency (0, 75, 150, 1000 µM Mg). Gradual decreases in growth and photosynthetic rates were observed with declining Mg concentrations and 0, 75, and 150 µM Mg increased oxidative stress as evidenced by H O and 2 2 lipid peroxidation. Total activities of SOD, CAT, POX, APX, GR, and GST were increased while NOX activity was decreased with Mg deficiency. The activities of GR and GST were highest in plants treated with 0 µM Mg, indicating excess use of glutathione for redox regulation. The most striking results were the changes in isoenzyme patterns of SOD, NOX, POX, and GST. For example, a new Cu/ ZnSOD isoenzyme was induced in plants treated with 0 µM Mg. Cotton plants adapt to Mg deficiency by ch...Continue Reading

References

Nov 1, 1971·Analytical Biochemistry·W WoodburyM A Stahman
Jul 12, 1973·Biochimica Et Biophysica Acta·C O Beauchamp, I Fridovich
Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Apr 1, 1968·Archives of Biochemistry and Biophysics·R L Heath, L Packer
Dec 1, 1984·Analytical Biochemistry·G RicciG Federici
May 31, 2001·Plant & Cell Physiology·S HiragaH Matsui
Jun 9, 2001·Phytochemistry·A P VitóriaR A Azevedo
Sep 21, 2004·Annual Review of Plant Biology·Klaus Apel, Heribert Hirt
Oct 7, 2004·Trends in Plant Science·Ron MittlerFrank Van Breusegem
Jun 29, 2005·Journal of Experimental Botany·Christian Hermans, Nathalie Verbruggen
Jan 10, 2006·Methods in Enzymology·Robert Edwards, David P Dixon
Sep 1, 1971·Plant Physiology·P M SeeversF F Catedral
Jun 13, 2006·Journal of Experimental Botany·John M Cheeseman
Aug 30, 2008·Physiologia Plantarum·Ismail Cakmak, Ernest A Kirkby
Nov 4, 2010·Plant Physiology·Christine H Foyer, Shigeru Shigeoka
Jan 6, 2011·Plant Physiology·Karl-Josef Dietz, Thomas Pfannschmidt
Jan 6, 2011·Plant Physiology·Christine H Foyer, Graham Noctor
Apr 13, 2011·Trends in Plant Science·Ron MittlerFrank Van Breusegem
Aug 31, 2012·Nature·Nathaniel D MuellerJonathan A Foley
Nov 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Boguslaw Stec

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