Mg(2+) differentially regulates two modes of mitochondrial Ca(2+) uptake in isolated cardiac mitochondria: implications for mitochondrial Ca(2+) sequestration

Journal of Bioenergetics and Biomembranes
Christoph A BlomeyerAmadou K S Camara

Abstract

The manner in which mitochondria take up and store Ca(2+) remains highly debated. Recent experimental and computational evidence has suggested the presence of at least two modes of Ca(2+) uptake and a complex Ca(2+) sequestration mechanism in mitochondria. But how Mg(2+) regulates these different modes of Ca(2+) uptake as well as mitochondrial Ca(2+) sequestration is not known. In this study, we investigated two different ways by which mitochondria take up and sequester Ca(2+) by using two different protocols. Isolated guinea pig cardiac mitochondria were exposed to varying concentrations of CaCl2 in the presence or absence of MgCl2. In the first protocol, A, CaCl2 was added to the respiration buffer containing isolated mitochondria, whereas in the second protocol, B, mitochondria were added to the respiration buffer with CaCl2 already present. Protocol A resulted first in a fast transitory uptake followed by a slow gradual uptake. In contrast, protocol B only revealed a slow and gradual Ca(2+) uptake, which was approximately 40 % of the slow uptake rate observed in protocol A. These two types of Ca(2+) uptake modes were differentially modulated by extra-matrix Mg(2+). That is, Mg(2+) markedly inhibited the slow mode of Ca(2+) ...Continue Reading

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Citations

Dec 6, 2017·Frontiers in Physiology·Shane AustinKarin Nowikovsky
Jan 8, 2019·PLoS Computational Biology·Sathyavani MalyalaJason N Bazil
Aug 2, 2017·Oxidative Medicine and Cellular Longevity·Ivana PilchovaPeter Racay
Sep 27, 2020·Antioxidants·Man Liu, Samuel C Dudley
Jan 6, 2021·Nutrients·Ligia DominguezMario Barbagallo
Jan 14, 2021·Nutrients·Ligia J DominguezMario Barbagallo
Jan 11, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Shane Austin, Karin Nowikovsky
Aug 15, 2017·Mitochondrion·Sabzali JavadovVladimir Makarov
Oct 27, 2021·Physiological Reviews·Joanne F Garbincius, John W Elrod

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