Mg(2+)-linked self-assembly of FtsZ in the presence of GTP or a GTP analogue involves the concerted formation of a narrow size distribution of oligomeric species.

Biochemistry
Begoña MonterrosoGermán Rivas

Abstract

The assembly of the bacterial cell division FtsZ protein in the presence of constantly replenished GTP was studied as a function of Mg(2+) concentration (at neutral pH and 0.5 M potassium) under steady-state conditions by sedimentation velocity, concentration-gradient light scattering, fluorescence correlation spectroscopy, and dynamic light scattering. Sedimentation velocity measurements confirmed previous results indicating cooperative appearance of a narrow size distribution of finite oligomers with increasing protein concentration. The concentration dependence of light scattering and diffusion coefficients independently verified the cooperative appearance of a narrow distribution of high molecular weight oligomers, and in addition provided a measurement of the average size of these species, which corresponds to 100 ± 20 FtsZ protomers at millimolar Mg(2+) concentration. Parallel experiments on solutions containing guanosine-5'-[(α,β)-methyleno]triphosphate, sodium salt (GMPCPP), a slowly hydrolyzable analogue of GTP, in place of GTP, likewise indicated the concerted formation of a narrow size distribution of fibrillar oligomers with a larger average mass (corresponding to 160 ± 20 FtsZ monomers). The closely similar behavio...Continue Reading

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Citations

Jul 13, 2012·The Journal of Biological Chemistry·Víctor M Hernández-RocamoraMiguel Vicente
May 7, 2013·Biophysics Reviews·Daniel Some
Sep 25, 2017·Biotechnology and Bioengineering·Adam W WestbrookC Perry Chou
Jul 16, 2013·The Journal of Biological Chemistry·Víctor M Hernández-RocamoraGermán Rivas
Aug 14, 2013·The Journal of Biological Chemistry·Rubén Ahijado-GuzmánGermán Rivas
Sep 4, 2015·The Journal of Biological Chemistry·Estefanía SalvarelliAna Isabel Rico
Jul 18, 2018·Nature Nanotechnology·Esra Te BrinkeWilhelm T S Huck
Jan 25, 2020·Current Biology : CB·Bryant E WalkerJaan Männik

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