MG53 Does Not Manifest the Development of Diabetes in db/db Mice

Diabetes
Qiang WangJianjie Ma

Abstract

MG53 is a member of the TRIM protein family that is predominantly expressed in striated muscles and participates in cell membrane repair. Controversy exists regarding MG53's role in insulin signaling and manifestation of diabetes. We generated db/db mice with either whole-body ablation or sustained elevation of MG53 in the bloodstream in order to evaluate the physiological function of MG53 in diabetes. To quantify the amount of MG53 protein in circulation, we developed a monoclonal antibody against MG53 with high specificity. Western blot using this antibody revealed lower or no change of serum MG53 levels in db/db mice or patients with diabetes compared with control subjects. Neither whole-body ablation of MG53 nor sustained elevation of MG53 in circulation altered insulin signaling and glucose handling in db/db mice. Instead, mice with ablation of MG53 were more susceptible to streptozotocin-induced dysfunctional handling of glucose compared with the wild-type littermates. Alkaline-induced corneal injury demonstrated delayed healing in db/db mice, which was restored by topical administration of recombinant human (rh)MG53. Daily intravenous administration of rhMG53 in rats at concentrations up to 10 mg/kg did not produce adver...Continue Reading

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Citations

Oct 3, 2020·Cardiovascular Toxicology·Wenhua JiangHeng Ma
Jan 14, 2021·Cell Biology International·Tingting WanYanbo Li
Feb 11, 2021·PloS One·Clothilde PhilouzePhilippe Delerive
Nov 27, 2020·Frontiers in Physiology·Dathe Z Benissan-MessanPeter H U Lee
May 31, 2021·Current Opinion in Pharmacology·Bryan A WhitsonJianjie Ma
Jun 30, 2021·Cell & Bioscience·Weina ZhongPeter H U Lee
Jul 10, 2021·Journal of Cellular and Molecular Medicine·Jinling DongLiqun Wang

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