Mga is sufficient to activate transcription in vitro of sof-sfbX and other Mga-regulated virulence genes in the group A Streptococcus

Journal of Bacteriology
Audry C AlmengorKevin S McIver

Abstract

The group A streptococcus (GAS), or Streptococcus pyogenes, is a strict human pathogen of medical significance, causing infections ranging from pharyngitis (strep throat) to necrotizing fasciitis (flesh-eating disease). Several virulence genes that encode factors important for colonization, internalization, and immune evasion are under the control of the multiple gene regulator of the GAS, or Mga. Mga functions as a DNA-binding protein that interacts with sites both proximal (Pemm and PscpA) and distal (PsclA) to the start of transcription for the genes that it regulates. The genes encoding serum opacity factor, sof, and a novel fibronectin-binding protein, sfbX, are cotranscribed and represent two uncharacterized Mga-regulated virulence genes in the GAS. Analysis of the promoter region of sof-sfbX identified a putative Mga-binding site 278 bp upstream of the regulated start of transcription as determined by primer extension. Electrophoretic mobility shift assays demonstrated that Mga is able to bind specifically to the single distal site in a fashion similar to the previously characterized PsclA. In order to better understand the events that take place at this and other Mga-regulated promoters, an in vitro transcription assay ...Continue Reading

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Citations

Oct 10, 2006·Journal of Bacteriology·Zerina KratovacDebra E Bessen
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Mar 28, 2008·Critical Reviews in Biochemistry and Molecular Biology·Milton H Saier
Nov 17, 2007·Molecular Microbiology·Samantha A Roberts, June R Scott
Sep 1, 2006·Molecular Microbiology·Anjuli M TimmerVictor Nizet
Nov 16, 2007·Molecular Microbiology·Elise R Hondorp, Kevin S McIver
May 9, 2013·Molecular Microbiology·Elise R HondorpKevin S McIver
Jan 25, 2011·Microbial Biotechnology·Monica MoschioniMichèle A Barocchi
Jul 28, 2005·International Journal of Medical Microbiology : IJMM·Gabriele Beyer-SehlmeyerAndreas Podbielski
Dec 21, 2016·Molecular Microbiology·Slawomir LukomskiRita Berisio
Dec 12, 2017·FEMS Microbiology Reviews·Hannah R FrostPierre R Smeesters

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