mGluR5 metabotropic glutamate receptor distribution in rat and human spinal cord: a developmental study

Neuroscience Research
A ValerioP Spano

Abstract

By combining biochemical, molecular and immunohistochemical approaches, we have investigated the presence of metabotropic glutamate receptors (mGluRs) belonging to the subtype 5 in rat and human spinal cords and the developmental changes in their expression. A polyclonal antibody raised against the carboxy-terminal portion of mGluR5 was used to study the distribution of the receptor in rat foetal (Et15), neonatal (P8) and adult spinal cords and dorsal root ganglia (DRG). mGluR5 appeared to be predominantly expressed in regions containing the primary sensory afferents. Immunoblotting with anti-mGluR5 antibody revealed lower receptor protein levels in rat adult spinal cord when compared with P8 rat spinal cord. Reverse transcriptase-polymerase chain reaction showed both mGluR5a and mGluR5b mRNAs expression in rat spinal cord. The mGluR5a variant was found more abundant in young animals than in adults. The pattern of mGluR5 immunostaining was also studied in foetal (6-8, 10, 12 and 22 weeks of gestation) and adult human spinal cord. At all stages of human development, a strong mGluR5 immunoreactivity was observed in the dorsal roots and in the dorsal and dorsolateral funiculi with maximum levels of staining at week 12 of gestation...Continue Reading

References

May 1, 1992·Pediatric Neurology·H B Sarnat
Nov 8, 1988·The Journal of Comparative Neurology·G Battaglia, A Rustioni
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Jan 1, 1994·Drugs·S T Meller, G F Gebhart
May 1, 1995·Neuroscience Research·P LiberiniP F Spano
Jun 1, 1995·Current Opinion in Neurobiology·J P Pin, J Bockaert
Jan 1, 1993·Trends in Neurosciences·S H Appel
Apr 28, 1995·Journal of Medicinal Chemistry·T KnöpfelH Allgeier
Jan 1, 1995·Journal of Neurochemistry·A CopaniF Nicoletti
Nov 6, 1995·The Journal of Comparative Neurology·A N van den PolP Ghosh
Nov 8, 1996·The Journal of Biological Chemistry·C RomanoK L O'Malley

❮ Previous
Next ❯

Citations

Jul 19, 2000·Brain Research·F Bordi, A Ugolini
Apr 9, 2002·European Journal of Pharmacology·Enza PalazzoSabatino Maione
Jul 16, 2002·Pharmacology, Biochemistry, and Behavior·Alessandra ValerioPierFranco Spano
Jul 16, 2002·Pharmacology, Biochemistry, and Behavior·Rosa Maria A SimmonsSmriti Iyengar
Jun 30, 2001·Trends in Pharmacological Sciences·W P SpoorenR Kuhn
Oct 19, 1999·Neurochemistry International·M C CuestaH Suarez-Roca
Jul 23, 1999·Progress in Neurobiology·F Bordi, A Ugolini
Jul 5, 2005·Nature Reviews. Neuroscience·Maria Fitzgerald
Oct 27, 2009·The Journal of Pain : Official Journal of the American Pain Society·Hui-Sheng ChenSu-Jie Lu
Oct 17, 2007·Pain·Erik LindströmL Ashley Blackshaw
Apr 17, 2002·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·James E HuettnerMin Zhuo
Jun 5, 2004·European Journal of Pharmacology·Enza PalazzoSabatino Maione
May 6, 2004·Brain Research. Developmental Brain Research·V D Di Giorgi GereviniF Nicoletti
May 29, 2007·Bioorganic & Medicinal Chemistry Letters·Xueqing WangAndrew O Stewart
Mar 28, 2012·Molecular Pain·Lara W CrockRobert W Gereau
Jun 22, 2000·The Journal of Comparative Neurology·F J AlvarezP M Somohano
Dec 28, 2017·International Journal of Molecular Sciences·Julia Fernández-MontoyaPilar Negredo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.