Abstract
A subline of the murine P815 mastocytoma passaged for a long period of time in histoincompatible hosts was found to be deficient in surface MHC class-I antigens by serological and biochemical methods. In agreement with the lack of restriction elements, this variant cell line was no longer susceptible to killing by cytotoxic T cells stimulated by and directed against the parental tumor cell line in syngeneic DBA-2 hosts. We did not observe the appearance of susceptibility to NK killing concomitantly with H-2 loss. We thus could not confirm the hypothesis of a regulatory function of H-2 structures in NK recognition/killing. Both cell lines were also resistant to lysis by mouse macrophages. The parental cell line was sensitive to rat macrophage killing, whereas the variant line had lost such sensitivity. In spite of resistance in vitro to various defense mechanisms, the variant H-2 loss tumor cell line was less tumorigenic in syngeneic hosts and exhibited a lower metastatic capacity than the parental cell line. We propose that in the H-2 loss subline, survival of cells in vivo is impeded since positive signals mediated by H-2 structures are missing, which are necessary for cell-cell contact and survival.
References
Jan 1, 1978·Journal of Immunological Methods·J W Goding
May 1, 1978·The Journal of Cell Biology·P F Bartlett, M Edidin
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A Harel-BellanD Fradelizi
Nov 15, 1985·Science·R S GoodenowR L Linsk
Aug 7, 1986·Nature·H Festenstein, F Garrido
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D W BahlerE M Lord
Sep 1, 1986·Cellular Immunology·R Keller, R Keist
May 23, 1985·Nature·R WallichM Feldman
Feb 20, 1986·Nature·K KärreR Kiessling
Apr 5, 1985·Science·K TanakaG Jay
Jan 1, 1974·Methods in Enzymology·M Morrison
Jan 1, 1984·Advances in Cancer Research·P C DohertyP J Wettstein
Jan 1, 1980·Immunobiology·M BertschmannE F Lüscher
Aug 1, 1957·Proceedings of the Society for Experimental Biology and Medicine·J MUNOZ
Dec 1, 1978·Immunogenetics·O J FinnH S Kaplan
Citations
Jul 1, 1996·Journal of Surgical Oncology·H HayashiM Imamura
Feb 20, 1992·International Journal of Cancer. Journal International Du Cancer·H M BlottièreJ Le Pendu
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ilia N BuhtoiarovPaul M Sondel
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y KawaradaG J Hämmerling