PMID: 9160098May 1, 1997Paper

MHC class I presentation of live and heat-inactivated Sendai virus antigen in T2Kb cells depends on an intracellular compartment with endosomal characteristics

Scandinavian Journal of Immunology
T LiuMikael Jondal

Abstract

T2Kb cells, which do not express TAP1/2 peptide transporters or the low molecular weight protein 2/7 (LMP2/7) proteasomal subunits, can still process and present both live and heat-inactivated Sendai virus (SV). As this operation may also reflect the existence of an alternative processing pathway in normal antigen-presenting cells (APC), the authors have characterized it using intracellular inhibitors and anti-Kb monoclonal antibodies (MoAbs). From the results with lipophilic amines (ammonium chloride, methylamine and chloroquine), cytoskeletal inhibitors (cytochalasin B and vinblastine), and an endoprotease inhibitor (phenylmethylsulfonyl fluoride, PMSF), the authors conclude that the processing of SV antigen in T2Kb cells has endosomal characteristics depending on cellular activities such as uptake, vesicular transport and intracellular-vesicular proteolysis. In addition, internalized 'empty' Kb molecules derived from the T2Kb cell surface appeared to be involved in the presentation of SV antigen, as demonstrated by a protocol using a combination of the Golgi inhibitor brefeldin A(BFA) and anti-Kb antibodies. The results thus indicate that T2Kb cells process SV antigen in an endosomal-like compartment which contain recycling ...Continue Reading

Citations

Dec 18, 2001·International Journal of Cancer. Journal International Du Cancer·R YangA Gruber
Feb 27, 2001·Journal of Immunological Methods·P K WallaceM W Fanger
Oct 18, 2002·Veterinary Immunology and Immunopathology·Silke RautenschleinJ M Sharma
Sep 10, 2003·Veterinary Immunology and Immunopathology·Matthew R Sandbulte, James A Roth
Aug 30, 2002·Molecular Immunology·Monique Grommé, Jacques Neefjes
Mar 10, 2004·Expert Opinion on Biological Therapy·Masoud H ManjiliJohn R Subjeck
Aug 18, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·N S Potter, C V Harding
Feb 19, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ralf M LeonhardtMichael R Knittler

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