PMID: 15383586Sep 24, 2004Paper

MHC class II molecules traffic into lipid rafts during intracellular transport

The Journal of Immunology : Official Journal of the American Association of Immunologists
Neil J PolosoPaul A Roche

Abstract

There have been many studies demonstrating that a portion of MHC class II molecules reside in detergent-insoluble membrane domains (commonly referred to as lipid rafts). We have proposed that the function of raft association is to concentrate specific MHC class II-peptide complexes in plasma membrane microdomains that can facilitate efficient T cell activation. We now show that MHC class II becomes lipid raft associated before binding antigenic peptides. Using pulse-chase radiolabeling techniques, we find that newly synthesized MHC class II and MHC class II-invariant chain complexes initially reside in a detergent-soluble membrane fraction and acquire detergent insolubility as they traffic to lysosomal Ag processing compartments. Monensin, an inhibitor of protein transport through the Golgi apparatus, blocks association of newly synthesized MHC class II with lipid rafts. Treatment of cells with leupeptin, which inhibits invariant chain degradation, leads to the accumulation of MHC class II in lipid rafts within the lysosome-like Ag-processing compartments. Raft fractionation of lysosomal membranes confirmed the presence of MHC class II in detergent-insoluble microdomains in Ag-processing compartments. These findings indicate th...Continue Reading

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Citations

Jan 31, 2006·Cellular Microbiology·Nicolas LapaqueJean-Pierre Gorvel
Oct 6, 2005·Journal of Leukocyte Biology·Claudia KaracsonyiRobert Lindner
Mar 4, 2006·The Journal of Biological Chemistry·Jason G KayJennifer L Stow
Aug 5, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kyung-Jin ChoPaul A Roche
Oct 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Neil J PolosoPaul A Roche
Mar 29, 2007·The Journal of Cell Biology·Fulvia VascottoAna-Maria Lennon-Duménil
Dec 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Juhan YoonHirohito Kita
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Dec 19, 2008·Journal of Cell Science·Adam C Berger, Paul A Roche
Dec 5, 2006·Current Opinion in Immunology·Fulvia VascottoAna-Maria Lennon-Duménil

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