PMID: 2497189Jun 1, 1989Paper

MHC class II transcription in different mouse cell types. Differential requirement for protein synthesis between B cells and macrophages

The Journal of Immunology : Official Journal of the American Association of Immunologists
J G WoodwardP M Stuart

Abstract

Although the MHC class II genes are known to be regulated transcriptionally, the relative rates of transcription of the four classical class II genes in different cell types have not been investigated. Using nuclear transcriptional analysis, we have investigated the transcriptional rates of the class II genes in the macrophage cell line WEHI-3, normal bone marrow-derived macrophages, L-929 cells, and two different B cell lymphoma lines. Kinetic analysis of class II transcription in IFN-gamma-treated WEHI-3 cells revealed a 4-h delay, followed by a rapid increase in transcription over the next 20 h. A significant basal level of class II transcription, apparent in bone marrow derived macrophages, was also further enhanced by IFN-gamma treatment. None of the class II genes were transcribed in L cells, whereas all class II genes were transcribed constitutively in the B cell lines. In both B cell lines and macrophages, the four class II genes were found to be transcribed at different rates from one another, but the only gene showing a consistent pattern in multiple experiments was A-alpha, always showing the highest rate. We also investigated the effect of protein synthesis inhibition on class II transcription. Cycloheximide treatme...Continue Reading

Related Concepts

Related Feeds

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
P M StuartJ G Woodward
The Journal of Immunology : Official Journal of the American Association of Immunologists
J XausA Celada
The Journal of Immunology : Official Journal of the American Association of Immunologists
P M StuartJ G Woodward
The Journal of Immunology : Official Journal of the American Association of Immunologists
M A McDowellD M Paulnock
© 2022 Meta ULC. All rights reserved