PMID: 9178812May 16, 1997Paper

MHC-dependent cytolysis of autologous tumor cells by lymphocytes infiltrating urothelial carcinomas

International Journal of Cancer. Journal International Du Cancer
F HousseauD Bellet

Abstract

Tumor-infiltrating lymphocytes (TIL) were grown from 23 urothelial carcinomas. Phenotyping analysis showed that the TIL cultures were mainly CD3+. Although CD4+ and CD8+ T-cell sub-sets were grown in culture, CD4+ T-cell sub-sets predominated over CD8+ T cells. Immunohistochemical studies performed on 5 tumor specimens confirmed this observation, and indicated that CD4+ T cells surrounded the tumor islets, whereas CD8+ T lymphocytes were localized among the tumor cells. Five short-term carcinoma cell lines established from these urothelial tumors were used as target cells in cytolysis assays in order to investigate the functional anti-tumor activity of autologous TIL. TIL from 4/5 tumors were lytic and 3 TIL lines displayed MHC-class-I-dependent cytotoxicity directed against autologous tumor cells. CD4+ T-cell-depletion experiments performed on TIL line 07 confirmed that CD8+ MHC-class-I-dependent CTL were the predominant effectors. Finally, experiments performed on 6 allogeneic urothelial-cancer cell lines matched for HLA-class-I molecules showed that TIL07 exhibited selective lytic activity toward tumor 07. These data indicate that CD8+ MHC-class-I-dependent CTL present in urothelial carcinomas are functional and may particip...Continue Reading

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Citations

Oct 22, 2013·EMBO Molecular Medicine·Mohamed El BehiClotilde Théry
Aug 9, 2016·Clinical Oncology : a Journal of the Royal College of Radiologists·R Leibowitz-AmitR Berger
Feb 22, 2002·International Journal of Cancer. Journal International Du Cancer·Virginie DanglesDominique Bellet
Jan 25, 2011·Journal of Experimental & Clinical Cancer Research : CR·Caigan Du, Yuzhuo Wang
Sep 20, 2018·Oncoimmunology·Michael PochShari Pilon-Thomas

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