MHC expression in a human adult stem cell line and its down-regulation by hCMV US gene transfection

The International Journal of Biochemistry & Cell Biology
Jae Young KimCurie Ahn

Abstract

Due to their unique capacity to self-renew and for multiple differentiation, stem cells are considered promising candidates for cell replacement therapy in many devastating diseases. However, studies on immune rejection, which is a major problem facing successful stem cell therapy, are rare. In this study, we examined MHC expression in the M13SV1 cell line, which has previously been shown to have stem cell properties and to be non-tumorigenic, in order to determine whether human adult stem cells might be rejected after transplantation. Our results show low expression levels of MHC class I molecules on the surface of these cells. An induction of MHC class I expression was observed when the cells were treated with IFN-gamma. Maximal induction of MHC class protein expression was observed at 48 h after treatment with concentrations above 5 ng/ml of IFN-gamma. Elevated MHC class I levels were sustained for 72 h after withdrawing IFN-gamma. Therefore, we introduced human cytomegalovirus (hCMV) US genes, which are known to be able to reduce MHC class I expression on the cell surface after infection, into M13SV1 cells. Cells transfected with the hCMV US2, US3, US6 or US11 genes exhibited a reduction (40-60%) of MHC class I expression c...Continue Reading

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Citations

Mar 24, 2006·Cardiology in the Young·Frank PillekampJürgen Hescheler
Feb 9, 2011·Immunology and Cell Biology·Daniëlle HorstEmmanuel J H J Wiertz
May 24, 2007·Tissue Engineering·Bonnie BarrilleauxKim C O'Connor
Apr 5, 2013·PloS One·Melisa A SolandGraça Almeida-Porada
Jun 19, 2008·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Peng-cheng ZhongYuan Lin
Sep 4, 2013·Regenerative Medicine·Graça Almeida-PoradaChristopher D Porada
Jun 23, 2011·Journal of Tissue Engineering and Regenerative Medicine·Ajit ThakurKim Jones

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