MHC expression kinetics and immunogenicity of mesenchymal stromal cells after short-term IFN-gamma challenge

Experimental Hematology
Wing Keung ChanGodfrey Chi-Fung Chan

Abstract

Under the influence of interferon-gamma (IFN-gamma), mesenchymal stromal cells (MSCs) are conditional antigen-presenting cells, which have immunosuppressive potential. Apart from IFN-gamma upregulation of major histocompatibility complexes class I and II (MHC-I and MHC-II) expression, the underlying kinetics and mechanisms have not been described previously. This information is helpful to delineate how human MSCs can be modulated by IFN-gamma in different clinical scenarios. Here, we demonstrated that IFN-gamma-treated MSCs underwent classical signal transduction pathway via phosphorylation of signal transducers and activators of transcription-1, activation of interferon regulatory factor-1, and class II transactivator comparable to that of primary human blood macrophages. IFN-gamma markedly induced expression of MHC-I instantly, while its effects on MHC-II were less dramatic and delayed up to 4 days. This is due to a slower intracellular transport of the MHC-II antigen to the membrane surface. More important is that MSCs showed a reduction in their proliferation by 50% without evidence of cell death after prolonged IFN-gamma treatment for 8 days. High-dose IFN-gamma-treated MSCs (500 U/mL) could initiate T-cell activation as i...Continue Reading

Citations

Dec 5, 2012·Immunology and Cell Biology·Matthew D GriffinThomas Ritter
Jul 19, 2011·Journal of Biomedical Science·Pei-Min ChenB-Linju Yen
Jan 10, 2013·Annual Review of Immunology·Paul S FrenetteChristoph Scheiermann
Nov 16, 2010·Annual Review of Pathology·Nora G Singer, Arnold I Caplan
Sep 6, 2011·Stem Cell Reviews and Reports·Mohamed AbumareeBill Kalionis
May 13, 2010·Journal of Biomedical Optics·Gobind DasEnzo Di Fabrizio
Aug 21, 2012·The International Journal of Biochemistry & Cell Biology·Yi LiuSongtao Shi
Apr 29, 2014·Anais Brasileiros De Dermatologia·Karolyn Sassi OgliariFabrizio Loth
Aug 15, 2014·Stem Cells Translational Medicine·Mimmi PatrikoskiSusanna Miettinen
Jan 25, 2018·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Mehdi NajarLaurence Lagneaux
Jun 11, 2017·Journal of Cellular Physiology·Elena R AndreevaLudmila B Buravkova
Jan 13, 2018·World Journal of Stem Cells·Larisa BroglieJeffrey A Medin
Jan 24, 2018·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Marc A SoaresDaniel J Ceradini
Oct 9, 2018·Journal of Periodontal Research·Karim M Fawzy El-SayedChristof E Dörfer
Dec 4, 2014·World Journal of Gastroenterology : WJG·Morgan VandermeulenOlivier Detry
Jan 14, 2020·Transplantation·Morgan VandermeulenFrançois Jouret
Nov 29, 2017·Stem Cells International·Cosette M Rivera-CruzMarxa L Figueiredo
Jul 30, 2020·Stem Cells International·Mengyuan WangHongzhi Fang

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