MhNCED3, a gene encoding 9-cis-epoxycarotenoid dioxygenase in Malus hupehensis Rehd., enhances plant tolerance to Cl- stress by reducing Cl- accumulation

Plant Physiology and Biochemistry : PPB
Wei-wei ZhangKun Ran

Abstract

High Cl(-) concentrations in tissues can be toxic to crop plants and may lead to reduced growth rates and yields. 9-cis-epoxycarotenoid dioxygenase (NCED) is thought to be involved in the biosynthesis of abscisic acid (ABA), which is an important regulator of plant adaptive responses to stress. Here, the expression of MhNCED3 in Malus hupehensis Rehd. and the effects of MhNCED3 on plant tolerance to Cl(-) stress were explored. The results showed that MhNCED3 expression and ABA biosynthesis in M. hupehensis Rehd. were induced by Cl(-) stress. Ectopic expression of MhNCED3 in Arabidopsis complemented the phenotypic defects of the 129B08/nced3 mutant and enhanced WT tolerance to Cl(-) stress. The transgenic Arabidopsis showed improved growth and developmental status, increased ABA contents, and reduced transpiration rates and relative water content. Furthermore, ectopic expression of MhNCED3 decreased Cl(-) accumulation and oxidative damage, and up-regulated the expression levels of AtCLCc (chloride channel protein) and AtSLAH3 (slow anion channel 1 homolog 3) genes in Arabidopsis. These observations suggest that MhNCED3 has critical role in enhancing plant tolerance to Cl(-) stress by reducing Cl(-) accumulation.

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Citations

Aug 28, 2015·Plant Physiology and Biochemistry : PPB·Wei-wei ZhangKun Ran
Feb 17, 2017·Proceedings of the National Academy of Sciences of the United States of America·Haiwen ZhangSheng Luan
Aug 5, 2017·Frontiers in Plant Science·Yanfen LuYuncong Yao
May 31, 2021·Plant, Cell & Environment·Cristina Martínez-AndújarFrancisco Pérez-Alfocea
Jul 23, 2021·Plant Physiology and Biochemistry : PPB·Meng LiHongyan Qi

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