Mi-flp-18 and Mi-mpk-1 Genes are Potential Targets for Meloidogyne incognita Control

The Journal of Parasitology
Linlin DongYuanmei Zuo

Abstract

Meloidogyne incognita is a major plant parasite that causes root-knot disease in numerous agricultural crops. This nematode has severely affected greenhouse crops in China. Chemical insecticides are generally used to control this pest, but they have adverse environmental and human toxicity effects; hence, safe and effective strategies for controlling the root-knot nematode (RKN) are necessary. FMRFamide-like peptides (FLPs) have diverse physiological and biological effects on the locomotory, feeding, and reproductive functions of nematodes, and mitogen-activated protein (MAP) kinase plays an important role in the regulation of transcription factors and protein kinases. These candidates are the common targets of RKN control. They are encoded by Mi-flp-18 and Mi-mpk-1 genes, respectively, in M. incognita . In this study, we used the RNA interference (RNAi) method to silence the transcription of these genes and determined the effects on the pathogenicity of RKN in potted plants. Real-time quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) revealed that Mi-mpk-1 gene expression could be reduced by 33% by RNAi. The RNAi-treated infective nematodes were inoculated with dsRNAs of Mi-flp-18 and Mi-mpk-1 in pot experi...Continue Reading

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Citations

Jan 15, 2017·Annals of Botany·François-Xavier GilletMaria Fatima Grossi-de-Sa
Jun 9, 2017·Bioscience, Biotechnology, and Biochemistry·Akito HosoiShinsaku Ito

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Datasets Mentioned

BETA
BE225475

Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

SPSS

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