PMID: 11916168Mar 28, 2002Paper

MICA is a target for complement-dependent cytotoxicity with mouse monoclonal antibodies and human alloantibodies

Human Immunology
Yizhou ZouPeter Stastny

Abstract

The highly polymorphic major histocompatibility class I related chain A (MICA) gene encodes glycoproteins that have been shown to be expressed in epithelial cells, endothelial cells, keratinocytes, monocytes, and tumor cells. In previous experiments, we have studied MICA antigens using rabbit sera obtained by immunization with MICA peptides. We also found that several transplant recipients had specific antibodies against MICA in an ELISA assay with recombinant of MICA (r-MICA). In the present work we produced monoclonal antibodies by immunization of mice with recombinant MICA*008. Based on the different patterns of reactivity observed in ELISA, Western blot, and flow cytometry, mAbs 1.9C2, 2.4F5, 1.7AD, and 2.3D4 only reacted with denatured MICA and mAb 1.7A8 and 3.2H3 reacted also with native MICA as illustrated by flow cytometry with live cells. These monoclonal antibodies were postulated to bind to different sites of the MICA molecule. In order to investigate whether MICA expressed on the cell surface is able to mediate cell killing, antibody absorption, flow cytometry and complement-dependent cytotoxicity (CDC) were performed. We found that mouse monoclonal antibody 3.2H3 was able to kill 70% of HeLa cells. Absorption of a ...Continue Reading

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Citations

Sep 28, 2007·The New England Journal of Medicine·Yizhou ZouGerhard Opelz
May 6, 2011·Transplantation·Qiuheng ZhangElaine F Reed
Dec 11, 2008·Immunogenetics·Yizhou ZouPeter Stastny
Dec 4, 2012·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Gabriela Franco SalinasDominique Baeten
Sep 2, 2015·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Elena Sánchez-ZapardielEstela Paz-Artal
Feb 16, 2010·Trends in Molecular Medicine·Mun-Kit Choy, Maude E Phipps
Aug 13, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Kazuo MizutaniJarhow Lee
Jun 9, 2004·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·R W M Collins
Jun 5, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Paul I Terasaki
Sep 23, 2008·Transplant International : Official Journal of the European Society for Organ Transplantation·Xiaobei LiKazunari Tanabe
May 1, 2011·Journal of Evidence-based Medicine·Jun LuYouping Li
Jan 1, 2014·Clinical Transplantation·Lei LuoHong Mei
Sep 14, 2010·Current Opinion in Immunology·Qiuheng Zhang, Elaine F Reed
Dec 18, 2004·Human Immunology·Steve TakemotoRene J Duquesnoy
Sep 19, 2006·Transplantation Proceedings·Z YeY Li
May 16, 2006·Human Immunology·Kazuo MizutaniJarhow Lee
May 16, 2006·Human Immunology·Yanzheng Zhang, Peter Stastny
Nov 22, 2005·Biochemical and Biophysical Research Communications·Jianqing ZhaoWei He
May 22, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·B Suárez-AlvarezC López-Larrea
Apr 22, 2011·Annals of Human Biology·V DurmanováM Buc
Jul 18, 2009·Current Opinion in Organ Transplantation·Yizhou Zou, Peter Stastny
Apr 28, 2006·Transplantation·Isabel QuirogaSusan V Fuggle
Feb 25, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yizhou ZouPeter Stastny
Jun 1, 2018·Journal of Immunology Research·Mohit ChowdhryVandana Sharma
Jun 11, 2021·International Journal of Immunogenetics·Christine NeuchelJoannis Mytilineos
Dec 13, 2006·Transplant Immunology·Núria AmézagaM G Ercilla
Jul 18, 2008·Transplantation Reviews·Luis Eduardo Morales-Buenrostro, Josefina Alberú

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