Abstract
We assess in mice, the electrophysiological criteria developed in humans and rats in vivo for unmyelinated (C) fibre differentiation into sub-classes, derived from the activity-induced latency increase ("slowing") in response to electrical stimulation during 6 min at 0.25 Hz followed by 3 min at 2 Hz. Fibres are considered nociceptors if they show more than 10% slowing at 2 Hz; nociceptors are further divided into mechanosensitive ("polymodal") and mechanoinsensitive ("silent") ones according to a latency shift of less and more than 1% during the first minute at 0.25 Hz, respectively. Sympathetic postganglionics are recognised by 2-10% slowing at 2 Hz; units slowing less than 2% at 2 Hz remain uncategorised. For assessment of these criteria, we also developed a novel in vivo technique for recording of peripheral single-fibres in the mouse. We compared the theoretical slowing-rate discriminator criteria with experimental data obtained from mice in vivo/in vitro and rats in vitro. Out of 69 cutaneous mouse C-fibres in vitro and 19 in vivo, only 38 (67%) and 9 (47%) met the above 1% criterion, respectively; sympathetics were not identified. In contrast, out of 20 rats nerve fibres in vitro, 19 (95%) met this criterion. We conclude...Continue Reading
References
Jan 1, 1988·Pain·James N CampbellSusan E Mackinnon
May 15, 1986·Neuroscience Letters·P W Reeh
Mar 8, 1974·Brain Research·H E Torebjörk, R G Hallin
Jan 1, 1995·Experimental Brain Research·M SchmelzH E Torebjörk
Oct 15, 1994·The Journal of Physiology·M SchmelzH E Torebjörk
Jan 1, 1994·Somatosensory & Motor Research·J G ThalhammerG R Strichartz
Aug 1, 1996·Neuroscience·M D GeeB Cotsell
Feb 5, 2003·Brain : a Journal of Neurology·Kristin ØrstavikErik Torebjörk
Sep 10, 2003·The Journal of Physiology·Hugh BostockJosé Ochoa
Jun 18, 1958·The Journal of Physiology·A IGGO
May 12, 2004·Neuroscience Letters·T SchlegelP W Reeh
Oct 7, 2006·The Journal of Physiology·Annette GeorgeHugh Bostock
Dec 22, 2007·The Journal of Physiology·Roberto De ColRichard W Carr
Jan 31, 2009·Nature Protocols·Katharina ZimmermannPeter W Reeh
Dec 7, 2011·The Journal of Physiology·Roberto De ColRichard W Carr
May 23, 2012·Trends in Pharmacological Sciences·Edward C EmeryPeter A McNaughton
Sep 17, 2013·Nature Genetics·Enrico LeipoldIngo Kurth
Feb 15, 2014·Pain·Sabine SchnorrStefan Herrmann
Aug 20, 2014·PloS One·Marcus E PeterssonErik Fransén
Citations
Apr 23, 2016·The Journal of Physiology·Tal HoffmannChristian Weidner
Jan 27, 2017·Journal of Neurophysiology·Roger H WatkinsRochelle Ackerley
Jun 21, 2019·Pain·Miriam M DüllBarbara Namer
Jan 17, 2020·Journal of Neural Engineering·N A Pelot, W M Grill
Mar 2, 2017·Physiological Research·P Dibaj, E D Schomburg