Mice deficient in nuclear factor (NF)-kappa B/p52 present with defects in humoral responses, germinal center reactions, and splenic microarchitecture

The Journal of Experimental Medicine
G FranzosoU Siebenlist

Abstract

p52 is a subunit of nuclear factor (NF)-kappa B transcription factors, most closely related to p50. Previously, we have shown that p52, but not p50 homodimers can form transactivating complexes when associated with Bcl-3, an unusual member of the I kappa B family. To determine nonredundant physiologic roles of p52, we generated mice deficient in p52. Null mutant mice were impaired in their ability to generate antibodies to T-dependent antigens, consistent with an absence of B cell follicles and follicular dendritic cell networks in secondary lymphoid organs, and an inability to form germinal centers. Furthermore, the splenic marginal zone was disrupted. These phenotypes are largely overlapping with those observed in Bcl-3 knockout animals, but distinct from those of p50 knockouts, supporting the notion of a physiologically relevant complex of p52 homodimers and Bcl-3. Adoptive transfer experiments further suggest that such a complex may be critical in accessory cell functions during antigen-specific immune reactions. Possible roles of p52 and Bcl-3 are discussed that may underlie the oncogenic potential of these proteins, as evidenced by recurrent chromosomal translocations of their genes in lymphoid tumors.

References

Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E N HatadaC Scheidereit
Mar 6, 1992·Cell·P MombaertsV E Papaioannou
Aug 1, 1991·British Journal of Anaesthesia·M O Columb
Jan 1, 1987·The Journal of Experimental Medicine·J P CoutelierJ Van Snick
Nov 11, 1994·Science·E W ShoresP E Love
Nov 15, 1995·Genes & Development·I M VermaS Miyamoto
Aug 1, 1994·Immunity·J XuR A Flavell
Jan 1, 1994·Annual Review of Cell Biology·U SiebenlistK Brown
Jan 1, 1994·Annual Review of Immunology·P A Baeuerle, T Henkel
Sep 14, 1994·Journal of Immunological Methods·P J LeenenW van Ewijk
Apr 1, 1994·Molecular and Cellular Biology·C E DabrowskiP A Schaffer
Jun 1, 1994·Molecular and Cellular Biology·Q ZhangT W McKeithan
Mar 1, 1996·The Journal of Experimental Medicine·E Stüber, W Strober
Apr 1, 1996·The Journal of Experimental Medicine·N ZamzamiG Kroemer
Jan 1, 1996·Annual Review of Immunology·A S Baldwin
Feb 1, 1996·Current Opinion in Genetics & Development·M Tewari, V M Dixit
Jan 1, 1996·Journal of Cellular Biochemistry·C F WareT L VanArsdale
Oct 1, 1996·The Journal of Experimental Medicine·Y J Liu, J Banchereau

❮ Previous
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Citations

Jul 14, 1999·Developmental Genetics·A MuggiaD Talarico
Nov 27, 2008·Cell Biochemistry and Biophysics·John F TremlMichael P Cancro
Aug 2, 2008·Immunologic Research·Christine A Goetz, Albert S Baldwin
Jan 22, 2004·Current Opinion in Immunology·Anne PuelJean-Laurent Casanova
Apr 10, 2004·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Rajani Ravi, Atul Bedi
Feb 13, 2004·Gene·Boris Bartholdy, Patrick Matthias
May 2, 2002·Cell·Sankar Ghosh, Michael Karin
Jul 11, 2003·Immunology Letters·Pascal Schneider, Jürg Tschopp
Jan 19, 2000·Immunology Today·S Marshall-ClarkeJ Hassan
Mar 14, 2003·Current Opinion in Immunology·Gerd Müller, Martin Lipp
Jun 25, 1998·Current Opinion in Immunology·D D Chaplin, Y Fu
May 14, 1999·Current Opinion in Immunology·M G McHeyzer-Williams, R Ahmed
Oct 18, 2003·Seminars in Immunology·Steve Gerondakis, Andreas Strasser
Aug 26, 2000·Cytokine & Growth Factor Reviews·A DenkB Baumann
Jun 6, 2003·Cytokine & Growth Factor Reviews·Paul W DempseyGenhong Cheng
Jun 6, 2003·Cytokine & Growth Factor Reviews·Gail A Bishop, Bruce S Hostager
Dec 25, 2002·Cancer Cell·Hans Häcker, Michael Karin
Nov 26, 2010·Cell Research·Vincent Feng-Sheng ShihAlexander Hoffmann
Jan 19, 2011·Cell Research·Matthew S Hayden, Sankar Ghosh
May 21, 2008·Immunology and Cell Biology·Micah J BensonRandolph J Noelle
Jul 31, 2012·Nature Immunology·Xiang XiaoXian Chang Li
May 30, 2006·Nature Immunology·Naozumi IshimaruJonathan Sprent
Aug 2, 2005·Nature Reviews. Immunology·Reina E Mebius, Georg Kraal
Feb 4, 2010·Nature Reviews. Nephrology·Antoine DurrbachBernard Charpentier
Jan 14, 2003·EMBO Reports·Jorge MoscatPaul Rennert
Oct 31, 2006·Oncogene·M S HaydenS Ghosh
Feb 27, 2010·The Journal of Biological Chemistry·Pradeep BistaMatvey E Lukashev
Sep 22, 1998·The Journal of Experimental Medicine·J D ChengR Bravo
Oct 18, 2000·The Journal of Experimental Medicine·A CariappaS Pillai
Feb 22, 2001·The Journal of Experimental Medicine·T KaishoS Akira
Aug 27, 2003·The Journal of Experimental Medicine·Deborah Veis NovackSteven L Teitelbaum
Feb 28, 2002·The Journal of Infectious Diseases·S ShapiraC A Hunter
Sep 22, 2006·Antioxidants & Redox Signaling·Cristen PantanoYvonne M W Janssen-Heininger

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Methods Mentioned

BETA
ubiquitination
transfection
ELISA
nuclear translocation
electrophoretic mobility shift

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