Mice develop effective but delayed protective immune responses when immunized as neonates either intranasally with nonliving VP6/LT(R192G) or orally with live rhesus rotavirus vaccine candidates.

Journal of Virology
J L VanCottR L Ward

Abstract

Rotavirus vaccines are delivered early in life, when the immune system is immature. To determine the effects of immaturity on responses to candidate vaccines, neonatal (7 days old) and adult mice were immunized with single doses of either Escherichia coli-expressed rotavirus VP6 protein and the adjuvant LT(R192G) or live rhesus rotavirus (RRV), and protection against fecal rotavirus shedding following challenge with the murine rotavirus strain EDIM was determined. Neonatal mice immunized intranasally with VP6/LT(R192G) were unprotected at 10 days postimmunization (dpi) and had no detectable rotavirus B-cell (antibody) or CD4(+) CD8(+) T-cell (rotavirus-inducible, Th1 [gamma interferon and interleukin-2 {IL-2}]-, Th2 [IL-5 and IL-4]-, or ThIL-17 [IL-17]-producing spleen cells) responses. However, by 28 and 42 dpi, these mice were significantly (P >or= 0.003) protected and contained memory rotavirus-specific T cells but produced no rotavirus antibody. In contrast, adult mice were nearly fully protected by 10 dpi and contained both rotavirus immunoglobulin G and memory T cells. Neonates immunized orally with RRV were also less protected (P=0.01) than adult mice by 10 dpi and produced correspondingly less rotavirus antibody. Both g...Continue Reading

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Citations

Feb 13, 2008·Expert Reviews in Molecular Medicine·Richard L Ward
Jun 16, 2007·Nature Reviews. Microbiology·Juana AngelHarry B Greenberg
Jun 3, 2010·Infection and Immunity·Andrea EcheverryBecky Adkins
Mar 10, 2011·Virology Journal·Karen KnippingBelinda Van't Land
Mar 4, 2014·Microbial Pathogenesis·Sayed-Mahdi MarashiZabihollah Shoja
Jan 30, 2010·Seminars in Immunopathology·Yinyao LinShabaana A Khader
Jan 5, 2013·Virus Research·Zabihollah ShojaRakhshandeh Nategh
Jun 16, 2010·Veterinary Immunology and Immunopathology·Diego Daniel GonzalezMaría José Dus Santos
Jul 29, 2009·Journal of Pediatric Surgery·Alexander J BondocGreg M Tiao
Dec 18, 2008·European Journal of Immunology·Alicia Demirjian, Ofer Levy
Dec 3, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Daniel E VelasquezBaoming Jiang
Dec 11, 2014·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Jayashree RavichandranCharani Ranasinghe
Mar 9, 2010·Journal of Biomaterials Applications·Bismita NayakPratima Ray
Nov 10, 2017·Nature Reviews. Disease Primers·Sue E CrawfordMary K Estes
Jan 8, 2019·Reviews in Medical Virology·Atefeh AfchangiZabihollah Shoja
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Nov 3, 2011·Expert Review of Vaccines·Luigi BuonaguroFranco M Buonaguro
Mar 9, 2012·Expert Review of Anti-infective Therapy·Eliana Akemi FutataMaria Notomi Sato
Jul 12, 2019·Journal of Virology·Laura María ReyManuel Antonio Franco
Jan 13, 2019·Nutrients·Maria Del Mar Rigo-AdroverFrancisco J Pérez-Cano
Dec 1, 2013·Microbiology Spectrum·Manuel A Franco, Harry B Greenberg

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