Mice Fed an Obesogenic Western Diet, Administered Antibiotics, and Subjected to a Sterile Surgical Procedure Develop Lethal Septicemia with Multidrug-Resistant Pathobionts

MBio
S K HyojuJ C Alverdy

Abstract

Despite antibiotics and sterile technique, postoperative infections remain a real and present danger to patients. Recent estimates suggest that 50% of the pathogens associated with postoperative infections have become resistant to the standard antibiotics used for prophylaxis. Risk factors identified in such cases include obesity and antibiotic exposure. To study the combined effect of obesity and antibiotic exposure on postoperative infection, mice were allowed to gain weight on an obesogenic Western-type diet (WD), administered antibiotics and then subjected to an otherwise recoverable sterile surgical injury (30% hepatectomy). The feeding of a WD alone resulted in a major imbalance of the cecal microbiota characterized by a decrease in diversity, loss of Bacteroidetes, a bloom in Proteobacteria, and the emergence of antibiotic-resistant organisms among the cecal microbiota. When WD-fed mice were administered antibiotics and subjected to 30% liver resection, lethal sepsis, characterized by multiple-organ damage, developed. Notable was the emergence and systemic dissemination of multidrug-resistant (MDR) pathobionts, including carbapenem-resistant, extended-spectrum β-lactamase-producing Serratia marcescens, which expressed a ...Continue Reading

References

Feb 4, 2006·The Journal of Trauma·Kentaro ShimizuHisashi Sugimoto
Oct 13, 2009·Expert Review of Pharmacoeconomics & Outcomes Research·Patricia W Stone
Mar 30, 2010·Journal of the American College of Surgeons·Emily WaisbrenElof Eriksson
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Jun 24, 2010·JAMA : the Journal of the American Medical Association·Janet M TorpyRichard M Glass
Aug 3, 2010·Molecular and Cellular Biochemistry·Kalpana BallalHeinrich Taegtmeyer
Nov 5, 2010·PloS One·Massimo AndreattaOle Lund
Feb 16, 2011·Immunology·Jonathan P Huber, J David Farrar
Mar 9, 2011·Digestive Diseases and Sciences·Mineji HayakawaSatoshi Gando
May 24, 2012·MBio·Thierry PédronPhilippe J Sansonetti
May 24, 2012·The Journal of Antimicrobial Chemotherapy·Keith Poole
Nov 6, 2012·Trends in Immunology·Mercedes B FuertesThomas F Gajewski
Sep 14, 2013·Critical Care : the Official Journal of the Critical Care Forum·Jason PhuaAmartya Mukhopadhyay
Oct 1, 2013·Critical Care : the Official Journal of the Critical Care Forum·Nicolas de ProstChristian Brun-Buisson
Dec 24, 2013·Nature Reviews. Immunology·Lionel B Ivashkiv, Laura T Donlin
Jun 22, 2014·Advances in Experimental Medicine and Biology·Mouna MarrakchiSilvana Andreescu
Oct 22, 2014·Antimicrobial Agents and Chemotherapy·Dušanka Djorić, Christopher J Kristich
Jul 22, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S ThelwallT Lamagni
Dec 20, 2015·The Journal of Clinical Endocrinology and Metabolism·Rebecca A HaeuslerEle Ferrannini
Mar 27, 2016·Critical Care Clinics·Nathan J Klingensmith, Craig M Coopersmith
Jun 21, 2016·Cell Metabolism·Annika WahlströmFredrik Bäckhed
Jun 29, 2016·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Hiroyuki TominagaSetsuro Komiya
Sep 13, 2016·Chest·Shipra GuptaKianoush B Kashani
Dec 14, 2016·Molecular Medicine Reports·Daniela CălinaEliza Gofita
Dec 17, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Alexander ZaborinJohn C Alverdy
Jan 26, 2017·The British Journal of Surgery·J C AlverdyJ A Gilbert
Apr 14, 2017·Surgical Infections·Elizabeth SullivanCharles H Cook
Apr 14, 2017·The Lancet. Gastroenterology & Hepatology·Bastiaan W Haak, W Joost Wiersinga
Apr 26, 2017·Nature Microbiology·Aaron G Wexler, Andrew L Goodman
Jun 3, 2017·Bio-protocol·Lian-Qun QiuPerry J Blackshear
Sep 13, 2017·Antimicrobial Agents and Chemotherapy·Anna KnöppelDan I Andersson
Oct 19, 2017·Frontiers in Medicine·Verónica Urdaneta, Josep Casadesús
Feb 10, 2018·Shock·Mei MengCraig M Coopersmith

❮ Previous
Next ❯

Citations

Apr 21, 2020·Surgical Infections·Jasper B van PraaghJohn C Alverdy
Oct 9, 2019·MBio·Christine M Bassis
Apr 23, 2020·Surgical Infections·Hannah Zhao-FlemingSharmila Dissanaike
Jun 24, 2020·Infection and Immunity·John C AlverdyRenee Thewissen
Sep 15, 2020·Journal of Alzheimer's Disease : JAD·Jade de OliveiraAndreza F de Bem
Mar 25, 2020·Critical Care : the Official Journal of the Critical Care Forum·Matteo BassettiAndrea Gori
Jun 4, 2020·Critical Care : the Official Journal of the Critical Care Forum·Max W AdelmanGreg S Martin
Oct 22, 2020·BMC Genomics·Houriiyah TegallyTulio de Oliveira
Dec 18, 2020·The Journal of Infectious Diseases·William D MillerJohn C Alverdy
Jul 7, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Eréndira Guadalupe Pérez-HernándezJaime Mas-Oliva

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJEB32897

Methods Mentioned

BETA
PCR
reverse transcription-PCR
flow cytometry
Phyloseq
protein assay

Software Mentioned

ANCOM
R
MicrobiomeSeq
opm
OpenTrons
analysis of composition of microbiome ( ANCOM )
Phyloseq
LASAF
Fiji
Prism

Related Concepts

Related Feeds

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.