PMID: 9182681Jun 16, 1997Paper

Mice genetically hyporesponsive to lipopolysaccharide (LPS) exhibit a defect in endocytic uptake of LPS and ceramide

The Journal of Experimental Medicine
N Thiéblemont, S D Wright

Abstract

We have recently shown that monomeric bacterial LPS is rapidly delivered from the plasma membrane to an intracellular site and that agents that block vesicular transport block responses of neutrophils to lipopolysaccharide (LPS) (Detmers, P.A., N. Thiéblemont, T. Vasselon, R. Pironkova, D.S. Miller, and S.D. Wright. 1996. J. Immunol. 157:5589-5596). To examine further the connection between intracellular transport of LPS and signaling, we observed internalization of fluorescently labeled LPS in cells from LPS-hyporesponsive (Lpsd) mice. Binding of fluorescent LPS from LPS-soluble CD14 (sCD14) complexes by peritoneal macrophages from Lpsd and control (Lpsn) mice was quantitatively similar, and confocal images obtained from these cells exhibited an identical appearance immediately after labeling. Incubation of labeled Lpsn macrophages at 37 degrees C caused movement of the fluorescence from the cell perimeter in one or two spots in the perinuclear region. However, in Lpsd cells the fluorescence remained dispersed, suggesting a defect in vesicular transport. LPS resembles ceramide, and Lpsd mice fail to respond to ceramide. As with LPS, we found that binding of fluorescent ceramide by Lpsd and Lpsn macrophages was quantitatively s...Continue Reading

References

May 10, 1985·Science·N G Lipsky, R E Pagano
Jan 1, 1994·The Journal of Experimental Medicine·E HailmanS D Wright
Jun 1, 1995·Immunology Today·S D Wright, R N Kolesnick
Feb 23, 1996·The Journal of Biological Chemistry·B Yu, S D Wright

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Citations

May 26, 1999·Infectious Disease Clinics of North America·R R IngallsD Golenbock
Sep 16, 2010·Expert Reviews in Molecular Medicine·Caroline JunqueiraRicardo T Gazzinelli
Aug 17, 1999·The Journal of Experimental Medicine·T VasselonP A Detmers
Aug 17, 1999·The Journal of Experimental Medicine·N Thieblemont, S D Wright
Mar 6, 2002·The Journal of Experimental Medicine·Mathias W HornefAgneta Richter-Dahlfors
Oct 22, 2003·The Journal of Experimental Medicine·Mathias W HornefStaffan Normark
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·C A ByrdA Ding
Dec 9, 2004·Immunology Letters·Matilde Leon-PonteJoaquín Madrenas
Aug 20, 2014·Communicative & Integrative Biology·Vicky Sender, Cordula Stamme
Mar 10, 2011·PloS One·Karine Narbonne-ReveauJulien Royet
May 17, 2012·PLoS Pathogens·Raquel Conde-ÁlvarezJean-Pierre Gorvel
Jun 2, 2012·PLoS Pathogens·Santosh K PandaBalachandran Ravindran

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