Mice genotyping using buccal swab samples: an improved method

Biochemical Genetics
Dinko MitrecićSrećko Gajović

Abstract

Routine methods used to genotype mice involve isolation of DNA from partially amputated neonate's tail, toe, or ear. The inevitable drawbacks of such techniques are the animal's pain response and the increased time and funds required for DNA purification. In order to implement a noninvasive and simple protocol for mouse DNA isolation, we have improved the method based on samples collected by swabbing of the inner cheek. Combining alkaline and temperature lysis, it was possible to isolate a DNA solution ready for PCR in less than an hour. Testing the method on three different mouse lines showed that it is highly efficient, the volume of the PCR samples could be reduced to 25 microl, and fragments up to 800 bp were successfully amplified. This protocol reduces animal discomfort, shortens the time for DNA isolation, and enables amplification of larger DNA fragments with optimal success rate, thus considerably facilitating large-scale genotyping of different mouse lines.

References

Jun 30, 2000·Biotechnology Annual Review·P G LantzP Rådström
May 22, 2003·Tuberculosis·E Schneider, K G Castro
Oct 31, 2003·Cancer Detection and Prevention·Wei CaoZuo Feng Zhang
Oct 14, 2004·Laboratory Animals·M MeldgaardB Finsen
May 9, 2006·Molecular Genetics and Metabolism·Y-H ZhangE R B McCabe

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Citations

Sep 8, 2015·Experimental Gerontology·Samantha J AlperJames M Harper
Oct 9, 2009·Genetic Testing and Molecular Biomarkers·Bozica Vrabec BranicaSrećko Gajović
May 18, 2019·Omics : a Journal of Integrative Biology·Evangelos BalafasNikolaos Kostomitsopoulos
Mar 21, 2012·Lab Animal·Zhidong ChenHideko Takahashi

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