Mice lacking 25OHD 1alpha-hydroxylase demonstrate decreased epidermal differentiation and barrier function

The Journal of Steroid Biochemistry and Molecular Biology
D D BikleP M Elias

Abstract

Keratinocytes express high levels of 25OHD 1alpha-hydroxylase (1OHase). The product of this enzyme, 1,25(OH)(2)D, promotes the differentiation of keratinocytes in vitro. To test whether 1OHase activity is essential for keratinocyte differentiation in vivo we examined the differentiation process in mice null for the expression of the 1alphaOHase gene (1alphaOHase(-/-)) by light and electron microscopy, by immunocytochemistry for markers of differentiation, by ion capture cytochemistry for calcium localization, and by function using transepidermal water loss (TEWL) to assess barrier integrity. Levels of involucrin, filaggrin, and loricrin-markers of differentiation in the keratinocyte and critical for the formation of the cornified envelope-were reduced in the epidermis of 1alphaOHase(-/-) mice. Calcium in the outer epidermis was reduced with loss of the calcium gradient from stratum basale to stratum granulosum. TEWL was normal in the resting state, but following disruption of the barrier, 1alphaOHase(-/-) mice had a markedly prolonged recovery of barrier function associated with a reduction in lamellar body secretion and a failure to reform the calcium gradient. Thus 1,25(OH)(2)D is essential for normal epidermal differentiatio...Continue Reading

References

Apr 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J A McLaneN Abdelkader
Aug 1, 1986·The Journal of Clinical Investigation·D D BikleP Elias
Jun 1, 1985·The Journal of Investigative Dermatology·G K MenonP M Elias
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·D K PandaD Goltzman
Feb 20, 2002·The Journal of Investigative Dermatology·Zhongjion XieDaniel D Bikle
Nov 26, 2002·The Journal of Investigative Dermatology·Peter M EliasKenneth R Feingold
Apr 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Olivier DardenneRené St-Arnaud

❮ Previous
Next ❯

Citations

Mar 12, 2010·Archives of Dermatological Research·Yvonne DombrowskiJürgen Schauber
Jan 29, 2010·Journal of Bone and Mineral Metabolism·Daniel D Bikle
Feb 10, 2007·The Journal of Clinical Investigation·Jürgen SchauberRichard L Gallo
Jan 30, 2014·Anais Brasileiros De Dermatologia·Kleyton de Carvalho MesquitaIzelda Maria Carvalho Costa
Jan 30, 2010·Cellular and Molecular Life Sciences : CMLS·Marie-Claire MéchinMichel Simon
Jul 1, 2004·The Journal of Steroid Biochemistry and Molecular Biology·D D BikleZ Xie
Oct 16, 2012·Journal of the American Academy of Dermatology·Jean Y TangMaryam M Asgari
Sep 2, 2009·The Journal of Allergy and Clinical Immunology·Jürgen Schauber, Richard L Gallo
Mar 17, 2005·British Journal of Cancer·M J Larriba, A Muñoz
Jan 30, 2013·Molecular Carcinogenesis·Natalie NemazannikovaCrispin R Dass
Jun 25, 2008·Experimental Dermatology·Jürgen Schauber, Richard L Gallo
Jul 26, 2005·Acta Dermato-venereologica·Peter S FriedmannEugene Healy
Feb 27, 2019·Journal of Burn Care & Research : Official Publication of the American Burn Association·Yoon Soo ChoSuk Hoon Ohn
Dec 12, 2012·The Journal of Pharmacy and Pharmacology·Natalie NemazannikovaCrispin R Dass
Nov 8, 2014·Photochemistry and Photobiology·Erin M BurnsNabiha Yusuf
Nov 8, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Juliane LindnerGuido Heine
Oct 5, 2012·The British Journal of Dermatology·G HeineM Worm
Dec 23, 2014·Cell Biochemistry and Function·Sandra RiegerThomas S Lisse
Apr 4, 2015·Cancer Prevention Research·Clara Curiel-LewandrowskiH-H Sherry Chow

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved